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Journal of Dental Research
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Humans Immunized with Streptococcus mutans Antigens by Mucosal Routes

N.K. Childers*,1, G. Tong1, F. Li1, A.P. Dasanayake2, K. Kirk3 and S.M. Michalek4

1 Department of Pediatric Dentistry and
2 Oral Biology, School of Dentistry, Room 308, 1530 3rd Ave. South, University of Alabama at Birmingham, Birmingham, AL, USA 35294-0007;
3 Department of Biostatistics, School of Public Health, University of Alabama at Birmingham; and
4 Department of Microbiology, University of Alabama at Birmingham;


Figure 1
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  		Figure 1. Study design. The subjects were immunized via the IN or IT route with either soluble E-GTF or liposomal E-GTF (L-E-GTF). Subjects were randomly assigned to group AN (soluble E-GTF, IN, n = 6), AT (soluble E-GTF, IT, n = 5), BN (L-E-GTF, IN, n = 5), or BT (L-E-GTF, IT, n = 5). Samples of parotid saliva, nasal wash, and blood were collected on days indicated by vertical lines. {downarrow} indicates days of immunization.

 

Figure 2
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  		Figure 2. IgA anti-E-GTF antibody activity in nasal wash secretions from IN immunized subjects (top panel). Values are the mean ratio of IgA E-GTF/total IgA for samples collected before and after immunization with 125 µg of soluble E-GTF minus standard deviation (Group AN; -{blacktriangleup}-, n = 6) or L-E-GTF plus standard deviation (Group BN; -{diamondsuit}-, n = 5) on days 0 and 7. IgA anti-E-GTF antibody activity in nasal wash secretions from IN (-•-) and IT (-{blacksquare}-) immunized subjects (bottom panel). Values are the mean ratio of IgA E-GTF/total IgA (plus standard deviation) for samples collected before and after immunization with 125 µg S. mutans antigens (combined data from soluble E-GTF and L-E-GTF groups) on days 0 and 7. Mixed-model analysis resulted in a significant difference between responses in IN (n = 11) vs. IT (n = 10) groups (p < 0.05, see RESULTS).

 

Figure 3
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  		Figure 3. IgA anti-E-GTF antibody activity in parotid saliva from IN immunized subjects (top panel). Values are the mean ratio of IgA E-GTF/total IgA for samples collected before and after immunization with 125 µg of soluble E-GTF minus standard deviation (Group AN; -{blacktriangleup}-, n = 6) or L-E-GTF plus standard deviation (Group BN; -{diamondsuit}-, n = 5) on days 0 and 7. IgA anti-E-GTF antibody activity in parotid saliva from IN (-•-) and IT (-{blacksquare}-) immunized subjects (bottom panel). Values are the mean ratio of E-GTF-specific/total IgA anti-E-GTF activity in parotid saliva samples collected from IN (plus standard deviation) and IT (minus standard deviation) immunized subjects (combined data from soluble E-GTF and L-E-GTF groups). Mixed-model analysis resulted in a significant difference between responses in IN (n = 11) vs. IT (n = 10) groups (p < 0.05, see RESULTS).

 

Figure 4
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  		Figure 4. IgG (top panel) and IgA (bottom panel) anti-E-GTF antibody activity in serum from IN (-•-) or IT (-{blacksquare}-) immunized subjects. Values are the mean levels (ng/mL) of anti-E-GTF activity before and after immunization (plus or minus standard deviation for IN or IT immunization, respectively). Data are combined from soluble E-GTF and L-E-GTF groups. Mixed-model analysis resulted in no significant difference between responses in IN (n = 11) vs. IT (n = 10) groups for IgG and IgA (p > 0.05, see RESULTS).

 

Journal of Dental Research, Vol. 81, No. 1, 48-52 (2002)
DOI: 10.1177/154405910208100111
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