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Poisson Analysis of Streptococcal Bond-strengthening on Saliva-coated Enamel

¹ Department of Orthodontics, West China Hospital of Stomatology, State Key Laboratory of Oral Diseases, Sichuan University, 610041 Chengdu, China;
² Department of Biomedical Engineering, University Medical Center Groningen, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands; and
³ Department of Orthodontics, University Medical Center Groningen, University of Groningen, Hanzeplein 1, 9700 RB Groningen, The Netherlands

Correspondence: ^* h.c.van.der.mei{at}med.umcg.nl

The forces responsible for bond-strengthening in initial oral bacterial adhesion are unknown. Since Lifshitz-Van der Waals and electrostatic forces work instantaneously upon approach, it is hypothesized that bond-strengthening is governed by hydrogen bonding. Poisson analysis of adhesion forces observed during the retraction of bacterial probes from surfaces in atomic force microscopy can be used to analyze the nature of the adhesion forces. Streptococcal adhesion forces increased from about –0.7 to –10.3 nN when the contact time between cell surfaces and salivary films on enamel was increased from 0 to 120 sec. Initial and final adhesion forces were stronger for initial colonizers of tooth surfaces (S. mitis, S. sanguinis) than for later, more cariogenic, strains (S. sobrinus, S. mutans). Retraction curves after increased contact times showed minor peaks, representative of hydrogen bonds, and Poisson analyses indicated repulsive non-specific forces of around +0.3 nN and slightly more attractive hydrogen-bonding forces (–1.0 nN) for initial than for late colonizers (–0.8 nN).

Key Words: saliva adhesion • bacteria • enamel • bond strengthening • atomic force microscopy • streptococci • biofilm

Journal of Dental Research, Vol. 88, No. 9, 841-845 (2009)
DOI: 10.1177/0022034509342523


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