This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Goldberg, A.J. Articles by Kazemi, R.B. Search for Related Content PubMed PubMed Citation Articles by Goldberg, A.J. Articles by Kazemi, R.B. Social Bookmarking                         What's this?

Polypeptide-catalyzed Biosilicification of Dentin Surfaces

¹ Center for Biomaterials, Department of Reconstructive Sciences, MC-1615,
² Division of Endodontology, Department of Oral Health and Diagnostic Sciences, and
⁶ Division of Operative Dentistry, Department of Reconstructive Sciences, School of Dental Medicine, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT06030-1615, USA;
³ Department of Macromolecular Science and Engineering, Case Western Reserve University, Cleveland, OH, USA;
⁴ Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY, USA; and
⁵ Institute of Materials Science, University of Connecticut, Storrs, CT, USA

Correspondence: Goldberg{at}uchc.edu

In situ formation of mineral particles by biocatalysis would be advantageous for occluding dentin tubules to reduce permeability or for sealing of material-tooth interfaces. One approach would require that the peptide-catalyst remain functional on the dentin surface. Based on recent observations of retained activity on other surfaces, we hypothesized that poly(L-lysine) (PLL), an analog of the protein catalyst responsible for silica formation in primitive marine species, would remain functional on dentin. PLL was applied to dentin discs along with a pre-hydrolyzed silica precursor, tetramethyl orthosilicate (TMOS). Discs were analyzed microscopically (scanning electron microscopy, SEM) and chemically (x-ray photoelectron spectroscopy, XPS). The treated discs, but not the negative controls, exhibited partial distinct coating whose XPS survey was consistent with that of silica, demonstrating that the polypeptide was required and retained its mediating activity. Peptide-catalysts that mediate mineral formation can retain functionality on dentin, suggesting a wide range of preventive and treatment strategies.

Key Words: silica • morphology • dentin • tubules • permeability

Journal of Dental Research, Vol. 88, No. 4, 377-381 (2009)
DOI: 10.1177/0022034509333838


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?