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Journal of Dental Research
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Biomaterials & Bioengineering

Odontogenic Potential of Post-natal Oral Mucosal Epithelium

E. Nakagawa1,2, T. Itoh1, H. Yoshie2 and I. Satokata1,*

1 Division of Developmental Biology and
2 Division of Periodontology, Department of Oral Biological Sciences, Niigata University Graduate School of Medical and Dental Sciences, 2-5274 Gakkocho-dori, Niigata 951-8514, Japan

Correspondence: satokata{at}med.niigata-u.ac.jp

A bioengineered tooth would provide a powerful alternative to currently available clinical treatments. Previous experiments have succeeded in bioengineering teeth using tooth germs from animal embryos. However, the ultimate goal is to develop a technology which enables teeth to be regenerated with the use of autologous cells. To pursue this goal, we re-associated the palatal epithelium from young mice with the odontogenic dental mesenchyme and transplanted the re-associated tissues into mouse kidney capsules. Morphologically defined teeth were formed from the re-associated cultured palatal epithelial cell sheets from mice aged up to 4 wks, but no tooth was formed when the palatal epithelium from mice after 2 days of age was directly re-associated. Our results demonstrated that post-natal non-dental oral mucosal epithelium can be used as a substitute for dental epithelium, and that epithelial cell sheet improves the ability of the oral epithelium of older mice to differentiate into dental epithelium.

Key Words: tooth bioengineering • oral epithelium • cell sheet • enamel organ • odontoblast

Abbreviations: GFP, green fluorescent protein • E12.5, embryonic day 12.5 • DMEM, Dulbecco’s modified Eagle’s medium • FCS, fetal calf serum • DPBS, Dulbecco’s phosphate-buffered saline • wk, week • PBS, phosphate-buffered saline • EGF, epidermal growth factor • H&E, hematoxylin-eosin • CK-1, cytokeratin-1 • CK-13, cytokeratin-13 • CK-14, cytokeratin-14 • CK-19, cytokeratin-19 • P1, post-natal day 1 • AM, ameloblast • SR, stellate reticulum • SI, stratum intermedium • E, enamel • D, Dentin • PD, predentin • OD, odontoblast • P, dental pulp • HERS, Hertwig’s epithelial root sheath

Journal of Dental Research, Vol. 88, No. 3, 219-223 (2009)
DOI: 10.1177/0022034509333198
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