Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Husvik, C.
Right arrow Articles by Halstensen, T.S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Husvik, C.
Right arrow Articles by Halstensen, T.S.
Right arrowPubmed/NCBI databases
*Gene*GEO Profiles
*HomoloGene*UniGene
*Compound via MeSH
*Substance via MeSH
Medline Plus Health Information
*Oral Cancer
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Biological

PGE2 Production in Oral Cancer Cell Lines is COX-2-dependent

C. Husvik, C. Khuu, M. Bryne and T.S. Halstensen*

Laboratory for Mucosal Immunology (LMI), Department of Oral Biology, University of Oslo, P.b. 1052, Blindern, Oslo 0316, Norway

Correspondence: thalsten{at}odont.uio.no

It has been suggested that epithelial cyclooxyge-nase-2 (COX-2) promotes oral carcinogenesis and carcinoma malignancy through increased prostaglandin E2 (PGE2) production. Although oral squamous cell carcinomas (OSCC) often express COX-2, they may also produce PGE2 in a COX-1-dependent manner. We used 6 isolated cell lines to investigate which COX isoforms OSCC may use for PGE2 production. COX-1 and -2 expression patterns divided the 6 OSCC cell lines into 3 distinct groups: both COX isoforms low, only COX-1 high, or both COX isoforms high. Multicolor immunohistofluorescence staining confirmed the COX-expression profiles in organotypic 3D cultures and the COX-2 dominance in OSCC tumors. Epidermal growth factor (EGF) stimulation induced COX-2 (but not COX-1) expression and increased PGE2 production, which was attenuated by COX-2 (but not COX-1) specific inhibition or siRNA-mediated COX-2 gene knockdown. Thus, PGE2 production in OSCC cell lines was COX-2-dependent.

Key Words: oral squamous cell carcinoma • siRNA • prostaglandin E2 • immunohistofluorescence

Abbreviations: COX, cyclooxygenase • EGF, epidermal growth factor • EGFR, epidermal growth factor receptor • NSAID, non-steroidal anti-inflammatory drug • OSCC, oral squamous cell carcinoma • PG, prostaglandin • PGE2, prostaglandin E2 • PGES, prostaglandin E2 synthase.

Journal of Dental Research, Vol. 88, No. 2, 164-169 (2009)
DOI: 10.1177/0022034508329519
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?