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Journal of Dental Research
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BIOLOGICAL

Mineralized Tissue Formation by BMP2-transfected Pulp Stem Cells

X. Yang1,2, P.M. van der Kraan3, Z. Bian1, M. Fan1, X.F. Walboomers2 and J.A. Jansen2,*

1 Key Lab for Oral Biomedical Engineering of Ministry of Education and Department of Endodontics, School and Hospital of Stomatology, Wuhan University, Wuhan, Hubei Province, P.R. China;
2 Department of Periodontology and Biomaterials, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands; and
3 Experimental Rheumatology and Advanced Therapeutics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands

Correspondence: * J.Jansen{at}dent.umcn.nl

Previously, in vitro differentiation of odontoblasts was shown for dental pulp stem cells (DPSCs) transfected with bone morphogenetic protein-2 (Bmp2). For this study, we hypothesized that such cells also show potential for mineralized tissue formation in vivo. DPSCs were transfected with Bmp2 and seeded onto a ceramic scaffold. These complexes were cultured in medium without dexamethasone, and thereafter placed subcutaneously in nude mice for 1, 4, and 12 weeks. Samples were evaluated by histology and real-time PCR for osteocalcin, bone sialoprotein, dentin sialophosphoprotein, and dentin matrix protein 1. Results indicated that only the transfected DPSCs showed obvious mineralized tissue generation, and 12 weeks of implantation gave the highest percentage of mineralized tissue formation (33 ± 7.3% of implant pore area). Real-time PCR confirmed these results. In conclusion, Bmp2-transfected DPSCs effectively show mineralized tissue formation upon ectopic implantation.

Key Words: dental pulp stem cell • adenovirus • Bmp2 • gene transfection • in vivo implantation

Journal of Dental Research, Vol. 88, No. 11, 1020-1025 (2009)
DOI: 10.1177/0022034509346258
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