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Journal of Dental Research
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BIOLOGICAL

Odontoblast TRP Channels and Thermo/Mechanical Transmission

A.R. Son1,{dagger}, Y.M. Yang1,{dagger}, J.H. Hong1, S.I. Lee1, Y. Shibukawa2 and D.M. Shin1,*

1 Department of Oral Biology, Brain Korea 21 Project, Yonsei University College of Dentistry, 134 Sinchon-dong Seodaemon-gu, Seoul, 120-752, Korea; and
2 Department of Physiology, Tokyo Dental College, Chiba 261-8502, Japan

Correspondence: * dmshin{at}yuhs.ac

Odontoblasts function as mechanosensory receptors because of the expression of mechanosensitive channels in these cells. However, it is unclear if odontoblasts direct the signal transmission evoked by heat/cold or osmotic changes. This study investigated the effects of heat/cold or osmotic changes on calcium signaling and the functional expression of the thermo/mechanosensitive transient receptor potential (TRP) channels in primary cultured mouse odontoblastic cells, with the use of RT-PCR, fluorometric calcium imaging, and electrophysiology. TRPV1, TRPV2, TRPV3, TRPV4, and TRPM3 mRNA was expressed, but TRPM8 and TRPA1 mRNA was not. The receptor-specific stimulation of TRPV1-3 (heat-sensing receptors) and TRPV4/ TRPM3 (mechanic receptors) caused increases in the intracellular calcium concentration. Moreover, the channel activities of TRPV1-4 and TRPM3 were confirmed by a whole-cell patch-clamp technique. These results suggest that primary cultured mouse odontoblasts express heat/mechanosensitive TRP channels and play a role in the underlying mechanisms of thermo/mechanosensitive sensory transmission.

Key Words: TRP channels • odontoblasts • heat • mechanosensitive

Journal of Dental Research, Vol. 88, No. 11, 1014-1019 (2009)
DOI: 10.1177/0022034509343413
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