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Journal of Dental Research
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BIOMATERIALS & BIOENGINEERING

Periodontal Gene Transfer by Ultrasound and Nano/Microbubbles

R. Chen1, M. Chiba2, S. Mori3, M. Fukumoto4 and T. Kodama1,*

1 Molecular Delivery System Laboratory, Department of Biomedical Engineering, Graduate School of Biomedical Engineering, Tohoku University, 2–1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan;
2 Division of Oral Physiology, Graduate School of Dentistry, Tohoku University, Japan;
3 Tohoku University Hospital, Sendai, Japan; and
4 Department of Pathology, Institute of Development, Aging, and Cancer, Tohoku University, Japan

Correspondence: * kodama{at}bme.tohoku.ac.jp

A non-viral gene delivery approach with nano/microbubbles and ultrasound offers opportunities for targeting soft tissues for gene therapy. The periodontium is a complex structure comprised of hard (cementum, alveolar bone) and soft tissues (periodontal ligament, gingivae). We hypothesized that our established gene delivery method would allow the periodontal tissue to be targeted for transfection for gene therapy. Expression kinetics and sites of transfection sites with this approach were investigated in rat periodontal tissue. Bioluminescence imaging revealed that transient gene expression was induced at day 1 posttransfection, while confocal microscopy showed that gene expression was localized in the muscle cells of gingival tissues. These findings indicate that regular transfection with this approach results in high gene expression, facilitating gene therapy for periodontal disease involving alveolar bone resorption.

Key Words: gene therapy • ultrasound • membrane permeability • periodontal tissues • non-viral vector

Journal of Dental Research, Vol. 88, No. 11, 1008-1013 (2009)
DOI: 10.1177/0022034509346119
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