Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Data Supplement
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Tanimoto, K.
Right arrow Articles by Li, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tanimoto, K.
Right arrow Articles by Li, W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Biological

Reduced Amelogenin-MMP20 Interactions in Amelogenesis Imperfecta

K. Tanimoto1, T. Le1, L. Zhu1, H.E. Witkowska2, S. Robinson2, S. Hall2, P. Hwang3, P. DenBesten1 and W. Li1,*

1 Department of Orofacial Sciences and
2 Department of Cell and Tissue Biology, School of Dentistry, University of California at San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, USA; and
3 Department of Biochemistry and Biophysics, School of Medicine, University of California at San Francisco, CA, USA

Correspondence: * corresponding author, Wu.Li{at}ucsf.edu

Amelogenin with a proline 41 to threonine mutation (P41T) is hydrolyzed at a lower rate by matrix metalloproteinase 20 (MMP20), resulting in an inherited tooth enamel defect, amelogenesis imperfecta (AI). The aim of this study was to elucidate the effect of P41T on the interactions between amelogenin and MMP20, which may contribute to the formation of this type of AI. The interactions of a recombinant wild-type human amelogenin and its P41T mutant with recombinant human MMP20 were compared by substrate competition assay, pull-down assay, and surface plasmon resonance (SPR). The results showed that the binding of the P41T mutant amelogenin for MMP20 was significantly lower than that of wild-type amelogenin. Our study supports a model in which the P41T mutation reduces the interactions between amelogenin and MMP20, leading to decreased degradation of amelogenin by MMP20, and resulting in AI.

Key Words: amelogenin • matrix metalloproteinase 20 • protein interaction • tooth enamel • biomineralization • amelogenesis imperfecta

Journal of Dental Research, Vol. 87, No. 5, 451-455 (2008)
DOI: 10.1177/154405910808700516
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




Journal of Dental Research Call for Editor