Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here for more information

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Figures Only
Right arrow Free Full Text Free
Right arrow Free Full Text (Free PDF) Free
Right arrow Data Supplement
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Keijser, B.J.F.
Right arrow Articles by Crielaard, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Keijser, B.J.F.
Right arrow Articles by Crielaard, W.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

RAPID COMMUNICATION

Pyrosequencing analysis of the Oral Microflora of healthy adults

B.J.F. Keijser1, E. Zaura2, S.M. Huse3, J.M.B.M. van der Vossen1, F.H.J. Schuren1, R.C. Montijn1, J.M. ten Cate2 and W. Crielaard2,*

1 TNO Quality of Life, Business Unit Food and Biotechnology Innovations, Microbial Genomics Group, Zeist, The Netherlands;
2 Department of Cariology Endodontology Pedodontology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU Amsterdam, Louwesweg 1, 1066 EA Amsterdam, The Netherlands; and
3 Josephine Bay Paul Center, Marine Biological Laboratory, Woods Hole, MA, USA

Correspondence: * corresponding author, w.crielaard{at}acta.nl

A good definition of commensal microflora and an understanding of its relation to health are essential in preventing and combating disease. We hypothesized that the species richness of human oral microflora is underestimated. Saliva and supragingival plaque were sampled from 71 and 98 healthy adults, respectively. Amplicons from the V6 hypervariable region of the small-subunit ribosomal RNA gene were generated by PCR, pooled into saliva and plaque pools, and sequenced by means of the Genome Sequencer 20 system at 454 Life Sciences. Data were evaluated by taxonomic and rarefaction analyses. The 197,600 sequences generated yielded about 29,000 unique sequences, representing 22 taxonomic phyla. Grouping the sequences in operational taxonomic units (6%) yielded 3621 and 6888 species-level phylotypes in saliva and plaque, respectively. This work gives a radically new insight into the diversity of human oral microflora, which, with an estimated number of 19,000 phylotypes, is considerably higher than previously reported.

Key Words: pyrosequencing • microflora • plaque • saliva • diversity

Abbreviations: GS-20, Genome Sequencer 20 • OTU, Operational Taxonomic Unit • PCR, polymerase chain-reaction • 16S rDNA, small- subunit (16S) ribosomal deoxyribonucleic acid • UV, ultraviolet

Journal of Dental Research, Vol. 87, No. 11, 1016-1020 (2008)
DOI: 10.1177/154405910808701104
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
M. Hasegawa, T. Osaka, K. Tawaratsumida, T. Yamazaki, H. Tada, G. Y. Chen, S. Tsuneda, G. Nunez, and N. Inohara
Transitions in Oral and Intestinal Microflora Composition and Innate Immune Receptor-Dependent Stimulation during Mouse Development
Infect. Immun., February 1, 2010; 78(2): 639 - 650.
[Abstract] [Full Text] [PDF]

Home page
 JDRHome page
S. Filoche, L. Wong, and C.H. Sissons
Oral Biofilms: Emerging Concepts in Microbial Ecology
Journal of Dental Research, January 1, 2010; 89(1): 8 - 18.
[Abstract] [Full Text] [PDF]

Home page
 JDRHome page
J.F. Siqueira Jr. and I.N. Rocas
Diversity of Endodontic Microbiota Revisited
Journal of Dental Research, November 1, 2009; 88(11): 969 - 981.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
L. Nibali, N. Donos, and B. Henderson
Periodontal infectogenomics
J. Med. Microbiol., October 1, 2009; 58(10): 1269 - 1274.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
Y. Sun, Y. Cai, L. Liu, F. Yu, M. L. Farrell, W. McKendree, and W. Farmerie
ESPRIT: estimating species richness using large collections of 16S rRNA pyrosequences
Nucleic Acids Res., June 1, 2009; 37(10): e76 - e76.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
K. V. Voelkerding, S. A. Dames, and J. D. Durtschi
Next-Generation Sequencing: From Basic Research to Diagnostics
Clin. Chem., April 1, 2009; 55(4): 641 - 658.
[Abstract] [Full Text] [PDF]