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Responses of the Human Submandibular Artery to ACh and VIP

D. Stoji^1,*, S. Pei², M. Radenkovi³, J. Popovi-Roganovi¹, Z. Pei⁴ and L. Grbovi³

¹ Department of Pharmacology, Faculty of Stomatology, University of Belgrade, Dr. Subotia br. 8, 11 000 Belgrade;
² Department of Pharmacology, Medical Faculty, University of Ni;
³ Department of Pharmacology, Clinical Pharmacology and Toxicology, Medical Faculty, University of Belgrade; and
⁴ Department of Maxillofacial Surgery, Medical Faculty, University of Ni, Serbia and Montenegro

Correspondence: ^* corresponding author, miona-25{at}beotel.yu

Endothelial vasodilatory substances may play a central role in the local regulation of vascular tone. We hypothesized that these substances can mediate endothelium-dependent vasodilatory responses to acetylcholine (ACh) and vasoactive intestinal peptide (VIP) in the human submandibular artery. We evaluated the contributions of endothelial vasodilatory substances to vessel relaxation in response to ACh and VIP, using different inhibitors of endothelial vasodilation, the nitric oxide synthase inhibitor, the cyclo-oxygenase inhibitor, indomethacin, the potassium channel blocker, and 4-aminopyridine. ACh and VIP caused an endothelium- and concentration-dependent relaxation in this artery. ACh relaxation was completely blocked after the concomitant addition of N^G-nitro-L-arginine and indomethacin. The vasorelaxant effect of ACh was not influenced by 4-aminopyridine. VIP relaxation was almost completely abolished by 4-aminopyridine, and was partly inhibited by N^G-nitro-L-arginine, but was not affected by indomethacin. Thus, in the human submandibular artery, ACh and VIP produced endothelium-dependent vasodilation with different underlying mechanisms: release of nitric oxide (NO) and cyclo-oxygenase products for ACh, and release of NO and endothelium-derived hyperpolarizing factor for VIP.

Key Words: ACh • VIP • human submandibular artery

Journal of Dental Research, Vol. 86, No. 6, 565-570 (2007)
DOI: 10.1177/154405910708600615


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