Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Uehara, A.
Right arrow Articles by Takada, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Uehara, A.
Right arrow Articles by Takada, H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Biological

Functional TLRs and NODs in Human Gingival Fibroblasts

A. Uehara and H. Takada*

Department of Microbiology and Immunology, Tohoku University Graduate School of Dentistry, Sendai, 980-8575, Japan

Correspondence: * corresponding author, dent-ht{at}mail.tains.tohoku.ac.jp

Since human gingival fibroblasts are the major cells in periodontal tissues, we hypothesized that gingival fibroblasts are endowed with receptors for bacterial components, which induce innate immune responses against invading bacteria. We found clear mRNA expression of Toll-like receptors (TLR)1, TLR2, TLR3, TLR4, TLR5, TLR6, TLR7, TLR8, TLR9, MD-2, MyD88, NOD1, and NOD2 in gingival fibroblasts. Gingival fibroblasts constitutively expressed these molecules. Upon stimulation with chemically synthesized ligands mimicking microbial products for these receptors, the production of pro-inflammatory cytokines, such as interleukin (IL)-6, IL-8, and monocyte chemoattractant protein-1, was markedly up-regulated. Furthermore, the production of pro-inflammatory cytokines induced by TLR and NOD ligands was significantly inhibited by an RNA interference assay targeted to NF-{kappa}B. These findings indicate that these innate immunity-related molecules in gingival fibroblasts are functional receptors involved in inflammatory reactions in periodontal tissues, which might be responsible for periodontal pathogenesis.

Key Words: TLR • NOD1 • NOD2 • fibroblasts • innate immunity

Journal of Dental Research, Vol. 86, No. 3, 249-254 (2007)
DOI: 10.1177/154405910708600310
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J EndocrinolHome page
E. Correa-de-Santana, B. Frohlich, M. Labeur, M. Paez-Pereda, M. Theodoropoulou, J. L. Monteserin, U. Renner, and G. K Stalla
NOD2 receptors in adenopituitary folliculostellate cells: expression and function
J. Endocrinol., October 1, 2009; 203(1): 111 - 122.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
A. E. King, A. W. Horne, S. Hombach-Klonisch, J. I. Mason, and H. O.D. Critchley
Differential expression and regulation of nuclear oligomerization domain proteins NOD1 and NOD2 in human endometrium: a potential role in innate immune protection and menstruation
Mol. Hum. Reprod., May 1, 2009; 15(5): 311 - 319.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
S. Selleri, M. Palazzo, S. Deola, E. Wang, A. Balsari, F. M. Marincola, and C. Rumio
Induction of pro-inflammatory programs in enteroendocrine cells by the Toll-like receptor agonists flagellin and bacterial LPS
Int. Immunol., August 1, 2008; 20(8): 961 - 970.
[Abstract] [Full Text] [PDF]