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Journal of Dental Research
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*NITRIC OXIDE
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Biological

Nitric Oxide in Pulp Cell Growth, Differentiation, and Mineralization

R. Yasuhara1, T. Suzawa1, Y. Miyamoto1,*, X. Wang1,2, M. Takami1, A. Yamada1 and R. Kamijo1

1 Department of Biochemistry and
2 Department of Clinical Cariology and Endodontology, Showa University School of Dentistry, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan

Correspondence: * corresponding author, yoichim{at}dent.showa-u.ac.jp

Dental preparation sometimes causes transient congestion, edema, and necrosis of the pulp. We hypothesized that nitric oxide (NO) is involved in the pathophysiological changes in pulp after preparation. The mRNA and protein expression of the inducible isoform of NO synthase (iNOS) was examined in murine pulp after dental preparation. The effects of NO on the proliferation, mineralization, and apoptosis of pulp cells were also studied in vitro. We found that not only iNOS, but also mRNAs for alkaline phosphatase and plasma membrane glycoprotein-1, were expressed in the pulp after preparation. NOC-18, an NO donor, suppressed the proliferation of pulp cells without inducing cell death, whereas it promoted the mineralization of cells cultured in the presence of β-glycerophosphate, ascorbic acid, dexamethasone, and KH2PO4. Under these conditions, NOC-18 induced the apoptosis of pulp cells. These results suggest that NO regulates the growth, apoptosis, and mineralization of pulp cells.

Key Words: dental pulp • nitric oxide • proliferation • mineralization • apoptosis

Journal of Dental Research, Vol. 86, No. 2, 163-168 (2007)
DOI: 10.1177/154405910708600211
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