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Journal of Dental Research
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Biological

Activation of Adenosine Receptor on Gingival Fibroblasts

T. Hashikawa1, M. Takedachi1, M. Terakura1, S. Yamada1, L.F. Thompson2, Y. Shimabukuro1 and S. Murakami1,*

1 Department of Periodontology, Division of Oral Biology and Disease Control, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan; and
2 Oklahoma Medical Research Foundation, Immunobiology and Cancer Program, Oklahoma City, OK 73104, USA

Correspondence: * corresponding author, ipshinya{at}dent.osaka-u.ac.jp

CD73 (ecto-5'-nucleotidase) on human gingival fibroblasts plays a role in the regulation of intracellular cAMP levels through the generation of adenosine, which subsequently activates adenosine receptors. In this study, we examined the involvement of ecto-adenosine deaminase, which can be anchored to CD26 on human gingival fibroblasts, in metabolizing adenosine generated by CD73, and thus attenuating adenosine receptor activation. Ecto-adenosine deaminase expression on fibroblasts could be increased by pre-treatment with a lysate of Jurkat cells, a cell line rich in cytoplasmic adenosine deaminase. Interestingly, the cAMP response to adenosine generated from 5'-AMP via CD73 and the ability of 5'-AMP to induce hyaluronan synthase 1 mRNA were significantly decreased by the pre-treatment of fibroblasts with Jurkat cell lysate. This inhibitory effect was reversed by the specific adenosine deaminase inhibitor. These results suggest that ecto-adenosine deaminase metabolizes CD73-generated adenosine and regulates adenosine receptor activation.

Key Words: adenosine receptor • CD73 • ecto-adenosine deaminase

Journal of Dental Research, Vol. 85, No. 8, 739-744 (2006)
DOI: 10.1177/154405910608500810
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