This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Hatakeyama, J. Articles by Kulkarni, A.B. Search for Related Content PubMed PubMed Citation Articles by Hatakeyama, J. Articles by Kulkarni, A.B. Social Bookmarking                         What's this?

Amelogenin-mediated Regulation of Osteoclastogenesis, and Periodontal Cell Proliferation and Migration

¹ Functional Genomics Section and
² Cell Biology Section, Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, NIH, 30 Convent Drive, MSC 4395, Bldg. 30, Room 122, Bethesda, MD 20892, USA;
³ Cartilage Biology and Orthopedics Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD, USA; and
⁴ Department of Anatomy and Cell Biology, University of Pennsylvania School of Dental Medicine, Philadelphia, PA, USA

Correspondence: ^* corresponding author, ak40m{at}nih.gov

We previously reported that amelogenin isoforms M180 and leucine-rich amelogenin peptide (LRAP) are expressed in the periodontal region, and that their absence is associated with increased cementum defects in amelogenin-knockout (KO) mice. The aim of the present study was to characterize the functions of these isoforms in osteoclastogenesis and in the proliferation and migration of cementoblast/periodontal ligament cells. The co-cultures of wild-type (WT) osteoclast progenitor and KO cementoblast/periodontal ligament cells displayed more tartrate-resistant acid phosphatase (TRAP)-positive cells than the co-cultures of WT cells. The addition of LRAP to both co-cultures significantly reduced RANKL expression and the TRAP-positive cells. Proliferation and migration rates of the KO cementoblast/periodontal ligament cells were lower than those of WT cells and increased with the addition of either LRAP or P172 (a porcine homolog of mouse M180). Thus, we demonstrate the regulation of osteoclastogenesis by LRAP, and the proliferation and migration of cementoblast/periodontal ligament cells by LRAP and P172.

Key Words: amelogenins • LRAP • osteoclastogenesis • knockout mice

Journal of Dental Research, Vol. 85, No. 2, 144-149 (2006)
DOI: 10.1177/154405910608500206


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				J. Hatakeyama, S. Fukumoto, T. Nakamura, N. Haruyama, S. Suzuki, Y. Hatakeyama, L. Shum, C. W. Gibson, Y. Yamada, and A. B. Kulkarni Synergistic Roles of Amelogenin and Ameloblastin Journal of Dental Research, April 1, 2009; 88(4): 318 - 322. [Abstract] [Full Text] [PDF]

				Y. Yagi, N. Suda, Y. Yamakoshi, O. Baba, and K. Moriyama In vivo Application of Amelogenin Suppresses Root Resorption Journal of Dental Research, February 1, 2009; 88(2): 176 - 181. [Abstract] [Full Text] [PDF]

				Y Kabasawa, K Nagumo, Y Takeda, N Kawashima, N Okada, K Omura, A Yamaguchi, and K Katsube Amelogenin positive cells scattered in the interstitial component of odontogenic fibromas J. Clin. Pathol., July 1, 2008; 61(7): 851 - 855. [Abstract] [Full Text] [PDF]