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Biological

Roles of CLCA and CFTR in Electrolyte Re-absorption from Rat Saliva

K. Ishibashi1, J. Yamazaki2,*, K. Okamura3, Y. Teng1, K. Kitamura2 and K. Abe1

1 Departments of Functional Bioscience,
2 Physiological Science & Molecular Biology, and
3 Morphological Biology, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan

Correspondence: * corresponding author, junyama{at}college.fdcnet.ac.jp

A molecular basis for Cl– re-absorption has not been well-characterized in salivary ductal cells. Previously, we found strong expression of a rat homologue proposed to be Ca2+-dependent Cl– channels (rCLCA) in the intralobular ducts of the rat submandibular gland. To address the question as to whether rCLCA and cystic fibrosis transmembrane conductance regulator (CFTR) are involved in Cl– re-absorption, we evaluated the electrolyte content of saliva from glands pre-treated with a small interfering RNA (siRNA). Retrograde injection into a given submandibular duct of an siRNA designed to knock down either rCLCA or CFTR reduced the expression of each of the proteins. rCLCA and CFTR siRNAs significantly increased Cl– concentration in the final saliva during pilocarpine stimulation. These results represent the first in vivo evidence for a physiological significance of rCLCA, along with CFTR, in transepithelial Cl– transport in the ductal system of the rat submandibular gland.

Key Words: salivary • duct • re-absorption • chloride channel • siRNA

Journal of Dental Research, Vol. 85, No. 12, 1101-1105 (2006)
DOI: 10.1177/154405910608501207
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