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Immunogenicity and Persistence of a Targeted Anti-caries DNA Vaccine

Q.A. Xu^1,, F. Yu^2,, M.W. Fan^1,*, Z. Bian¹, Z. Chen¹, B. Fan¹, R. Jia¹ and J.H. Guo¹

¹ The Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, Luoyu Road 237, 430079 Wuhan, Hubei, China; and
² now at Center of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;

Correspondence: ^* corresponding author, kqyywjtx{at}public.wh.hb.cn

We have previously reported that a targeted anti-caries DNA vaccine, pGJA-P, induced accelerated and increased antibody responses compared with a non-targeted anti-caries DNA vaccine. Recently, pGJA-P/VAX, a new targeted anti-caries DNA vaccine for human trials, was constructed by replacing the pCI vector used in the construction of pGJA-P with pVAX1, the only vector authorized by the US Food and Drug Administration in clinical trials. Here, we report on our exploration of the kinetics of the antibody responses generated following pGJA-P/VAX immunization and the persistence of pGJA-P/VAX at both the inoculation site and the draining lymph nodes. Intranasal vaccination of mice with pGJA-P/VAX induced strong antibody responses that lasted for more than 6 months. Furthermore, pGJA-P/VAX could still be detected at both the inoculation site and the draining cervical lymph nodes 6 months after immunization. Thus, the persistent immune responses are likely due to the DNA depot in the host, which acts as a booster immunization.

Key Words: DNA vaccines • Streptococcus mutans • dental caries

Journal of Dental Research, Vol. 85, No. 10, 915-918 (2006)
DOI: 10.1177/154405910608501008


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