Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Kawashima, N.
Right arrow Articles by Suda, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kawashima, N.
Right arrow Articles by Suda, H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Biological

Effect of NOS Inhibitor on Cytokine and COX2 Expression in Rat Pulpitis

N. Kawashima1,*,3, H. Nakano-Kawanishi1, N. Suzuki1, M. Takagi2 and H. Suda1,3

1 Pulp Biology and Endodontics, Department of Restorative Sciences,
2 Molecular Pathology, Department of Oral Restitution, Division of Oral Health Sciences, Graduate School, Tokyo Medical & Dental University, and
3 Center of Excellence (COE) Program for Research on Molecular Destruction and Reconstruction of Tooth and Bone, Tokyo Medical & Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan;

Correspondence: * corresponding author, kawashima.n.endo{at}tmd.ac.jp

Various kinds of chemical mediators are synthesized in the course of pulpitis; thus, control of their production would assist in inducing a reduction in pulpal inflammation. We hypothesized that nitric oxide (NO) would be an important mediator of pulpal inflammation. Pulpal inflammation was induced by the application of LPS in rat incisor pulp, and inducible nitric oxide synthase (iNOS) expression was evaluated by reverse-transcription/polymerase chain-reaction and immunohistochemical staining. After LPS application, iNOS mRNA was first detected after 3 hrs, peaked at 6 hrs, and decreased thereafter. iNOS-positive cells were macrophages and neutrophils. An NOS inhibitor caused drastic decreases in the expression of pro-inflammatory cytokines and COX2 mRNA, which was highly induced in the LPS-induced pulpitis. These results indicate that NO synthesis is related to the initiation of mediator production, and that its down-regulation should contribute to the prevention of pro-inflammatory mediator synthesis. Abbreviations: ANOVA, analysis of variance; COX2, cyclo-oxygenase 2; EDTA, ethylenediaminetetraacetic acid; iNOS, inducible nitric oxide synthase; IL, interleukin; L-NAME, NG-nitro L-arginine methyl ester; LPS, lipopolysaccharide; NO, nitric oxide; NOS, nitric oxide synthases; PG, prostaglandin; RT-PCR, reverse-transcription/polymerase chain-reaction; TNF{alpha}, tumor necrosis factor alpha.

Key Words: nitric oxide synthase • pulpitis • cytokines • L-NAME • immunocompetent cells

Journal of Dental Research, Vol. 84, No. 8, 762-767 (2005)
DOI: 10.1177/154405910508400815
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Physiol.Home page
A. Bletsa, E. Berggreen, I. Fristad, O. Tenstad, and H. Wiig
Cytokine signalling in rat pulp interstitial fluid and transcapillary fluid exchange during lipopolysaccharide-induced acute inflammation
J. Physiol., May 15, 2006; 573(1): 225 - 236.
[Abstract] [Full Text] [PDF]