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Journal of Dental Research
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RAPID COMMUNICATION

Fusobacterium nucleatum Apoptosis-inducing Outer Membrane Protein

C.W. Kaplan1, R. Lux3, T. Huynh3, A. Jewett3, W. Shi1,2,3 and S. Kinder Haake3,*

1 Molecular Biology Institute,
2 Department of Microbiology, Immunology and Molecular Genetics, and
3 Department of Oral Biology and Oral Medicine, Dental Research Institute, Section of Periodontics, UCLA School of Dentistry, 10833 Le Conte Ave., Los Angeles, CA 90095-1668;

Correspondence: * corresponding author, shaake{at}dent.ucla.edu

The periodontal pathogen Fusobacterium nucleatum induces apoptosis in lymphocytes. We previously identified the autotransporter protein Fap2 in F. nucleatum strain PK1594 that induced apoptosis in lymphocytes when expressed in Escherichia coli. In this study, we identified protein homologs of Fap2 in the transformable F. nucleatum strain ATCC 23726, to determine their role in the induction of apoptosis in lymphocytes. We used a new gene-inactivation vector conferring thiamphenicol resistance (pHS31) to construct a mutant deficient in one of the homologs, aim1. Transcriptional analyses demonstrated disruption of aim1 expression, and phenotypic analyses revealed a 41% decrease in the ability of the mutant to induce apoptosis in Jurkat cells, as compared with the parental strain. These studies demonstrate, in the native host cell background, the contribution of aim1 to F. nucleatum induction of apoptosis and, to the best of our knowledge, represent the first report of a genetically defined and phenotypically characterized mutation in F. nucleatum.

Key Words: Fusobacterium nucleatum • apoptosis • autotransporter • aim1 • mutant

Journal of Dental Research, Vol. 84, No. 8, 700-704 (2005)
DOI: 10.1177/154405910508400803
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