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Journal of Dental Research
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Biological

PTH(1-34) Affects Osteoprotegerin Production in Human PDL Cells in vitro

S. Lossdörfer*, W. Götz and A. Jäger

Department of Orthodontics, Dental Clinic, University of Bonn, Welschnonnenstr. 17, 53111 Bonn, Germany;

Correspondence: * corresponding author, s.lossdoerfer{at}gmx.de

Since periodontal ligament (PDL) cells exhibit several osteoblastic traits, we hypothesized that human PDL cells will respond to hormonal stimulation in an osteoblast-like manner. Confluent and pre-confluent PDL cells from six patients were challenged with PTH(1-34). Cell number, ALP, osteocalcin, osteoprotegerin, and RANKL expression were determined. Intermittent PTH(1-34) treatment of confluent PDL cells caused a significant increase in proliferation, whereas differentiation and osteoprotegerin production decreased significantly. In pre-confluent PDL cells, this treatment regimen induced a biphasic decrease in proliferation, but a biphasic increase in differentiation and osteoprotegerin production. Continuous PTH(1-34) exposure enhanced proliferation but inhibited osteocalcin production in confluent cells and stimulated osteoprotegerin production in pre-confluent PDL cells. RANKL was hardly detectable and unaffected by PTH(1-34) treatment. These results indicate that human PDL cells respond to PTH(1-34) in an osteoblast-like manner, and that the PTH(1-34) effect depends on the maturation state of the cells and on the mode of administration.

Key Words: PTH • PDL cells • intermittent • continuous • OPG

Journal of Dental Research, Vol. 84, No. 7, 634-638 (2005)
DOI: 10.1177/154405910508400710
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