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Enamel Matrix Derivative Gel Stimulates Signal Transduction of BMP and TGF-β

¹ Department of Periodontics and Endodontics, and
² Department of Biochemistry, School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan;
³ Division of Pathophysiology, Research Center for Genomic Medicine, Saitama Medical School, 1397-1 Yamane Hidaka, Saitama 350-1241, Japan; and
⁴ Department of Biologic and Materials Science, School of Dentistry, University of Michigan, 1210 Eisenhower Place, Ann Arbor, MI 48108, USA;

Correspondence: ^* corresponding author, suzuki-shin{at}tsurumi-u.ac.jp

It has been shown that Emdogain^® Gel (Emd-Gel) containing enamel matrix proteins promotes biomineralization, such as osteogenesis and cementogenesis, during the regeneration of periodontal tissues. However, the growth factors involved in these activities of Emd-Gel remain unclear. In this study, Emd-Gel was fractionated into 22 sub-fractions by size exclusion chromatography. The osteoinductive factors, TGF-β and BMP, were examined by a specific luciferase reporter gene assay. In the unfractionated Emd-Gel, TGF-β-like activity was detected, while BMP activity was not. In contrast, in the fractionated Emd-Gel samples, TGF-β-like activity was detected from fractions 8 to 13, and BMP-like activity was detected from fractions 4 to 6. Also, it was confirmed that the BMP-like activity in Emd-Gel was inhibited by authentic TGF-β1 and TGF-β-like activity. These results indicate that Emd-Gel contains both TGF-β- and BMP-like growth factors that contribute to the induction of biomineralization during periodontal regeneration.

Key Words: enamel matrix derivative gel (Emdogain^®Gel) • transforming growth factor-β (TGF-β) • bone morphogenetic protein (BMP) • luciferase reporter assay

Journal of Dental Research, Vol. 84, No. 6, 510-514 (2005)
DOI: 10.1177/154405910508400605


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