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Journal of Dental Research
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Biological

Effects of Positive Pressure in Odontogenic Keratocysts

S. Oka1, Y. Kubota1,*, T. Yamashiro1, S. Ogata1, T. Ninomiya1, S. Ito2 and K. Shirasuna1

1 Department of Oral and Maxillofacial Surgery and 2 Department of Dental Anesthesiology, Graduate School of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan;

Correspondence: * corresponding author, yasu{at}dent.kyushu-u.ac.jp

Intracystic fluid pressure is thought to be involved in odontogenic cyst growth. In this study, we investigated the effects of positive pressure on the expression of interleukin-1{alpha} (IL-1{alpha}), matrix metalloproteinases (MMPs), and prostaglandin E2 (PGE2) in odontogenic keratocysts to determine whether this pressure stimulates inflammatory cytokine production and signaling of osteoclastogenic events. Positive pressure enhanced the expression of IL-1{alpha} mRNA and protein in odontogenic keratocyst epithelial cells, and increased the secretion of MMP-1, MMP-2, MMP-3, and PGE2 in a co-culture of odontogenic keratocyst fibroblasts and the epithelial cells. The pressure-induced secretions were inhibited by an interleukin-1 receptor antagonist. Recombinant human interleukin-1{alpha} (rhIL-1{alpha}) increased the secretion of MMP-1, MMP-2, MMP-3, and PGE2 in the fibroblasts. Furthermore, in the fibroblasts, rhIL-1{alpha} enhanced the expression of macrophage colony-stimulating factor (M-CSF) mRNA, and rhIL-1{alpha}-induced PGE2 increased the expression of nuclear factor {kappa}B ligand (RANKL) mRNA. Thus, positive pressure may play a crucial role in odontogenic keratocyst growth via stimulating the expression of IL-1{alpha} in epithelial cells.

Key Words: positive pressure • interleukin-1{alpha} • odontogenic keratocysts.

Journal of Dental Research, Vol. 84, No. 10, 913-918 (2005)
DOI: 10.1177/154405910508401008
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