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Journal of Dental Research
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Biological

Recovery of Stem Cells from Cryopreserved Periodontal Ligament

B.-M. Seo1,3, M. Miura1, W. Sonoyama1, C. Coppe1, R. Stanyon2 and S. Shi1,*

1 Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, Building 30, Room 131, 30 Convent Drive MSC-4320, and 2 Comparative Molecular Cytogenetics Core, Genetics Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA; and 3 Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University, Seoul, Korea;

Correspondence: * corresponding author, sshi{at}dir.nidcr.nih.gov

Human post-natal stem cells possess a great potential to be utilized in stem-cell-mediated clinical therapies and tissue engineering. It is not known whether cryopreserved human tissues contain functional post-natal stem cells. In this study, we utilized human periodontal ligament to test the hypothesis that cryopreserved human periodontal ligament contains retrievable post-natal stem cells. These cryopreserved periodontal ligament stem cells maintained normal periodontal ligament stem cell characteristics, including expression of the mesenchymal stem cell surface molecule STRO-1, single-colony-strain generation, multipotential differentiation, cementum/periodontal-ligament-like tissue regeneration, and a normal diploid karyotype. Collectively, this study provides valuable evidence demonstrating a practical approach to the preservation of solid-frozen human tissues for subsequent post-natal stem cell isolation and tissue regeneration. The present study demonstrates that human post-natal stem cells can be recovered from cryopreserved human periodontal ligament, thereby providing a practical clinical approach for the utilization of frozen tissues for stem cell isolation.

Key Words: periodontal ligament stem cells (PDLSCs) • osteoblast • cementum • cryopreservation

Journal of Dental Research, Vol. 84, No. 10, 907-912 (2005)
DOI: 10.1177/154405910508401007
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