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Journal of Dental Research
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Biological

Hyper-expression of Osteocalcin mRNA in Odontoblasts of Hyp Mice

T. Onishi1, T. Ogawa1, T. Hayashibara1, T. Hoshino1,2, R. Okawa1 and T. Ooshima1,*

1 Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565-0871, Japan; and
2 Division of Pediatric Dentistry, Department of Developmental and Reconstructive Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1, Sakamoto, Nagasaki, 852-8588, Japan;

Correspondence: * corresponding author, ooshima{at}dent.osaka-u.ac.jp

The Hyp mouse is a murine homologue of human X-linked hypophosphatemia that displays hypo-mineralization in bone and dentin. In this study, we tested the hypothesis that the defect in Hyp mice leads to alterations in the expression of dentin matrix proteins that may be associated with the hypo-mineralization changes in the tissues. Quantitative RT-PCR analyses showed that expression of the osteocalcin gene in Hyp mice tooth germ samples was significantly higher than in wild-type mice, whereas the gene expressions of osteonectin, osteopontn, dentin matrix protein 1, and type I collagen in both types of mice were similar. Further, cultured Hyp mice tooth germ samples exhibited a higher expression of the osteocalcin gene than did those from wild-type mice, which was in accord with the results of our in vivo analysis. These findings suggest that osteocalcin mRNA is highly expressed in Hyp mice odontoblasts and may be associated with dentin hypo-mineralization.

Key Words: dentinogenesis • osteocalcin • real-time RT-PCR • X-linked hypophosphatemic vitamin-D-resistant rickets

Journal of Dental Research, Vol. 84, No. 1, 84-88 (2005)
DOI: 10.1177/154405910508400115
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