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Journal of Dental Research
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Biological

Macrophages and Mast Cells Control the Neutrophil Migration Induced by Dentin Proteins

T.A. Silva1,2,*, V.S. Lara1, J.S. Silva3, S.H.P. Oliveira4, W.T. Butler5 and F.Q. Cunha2

1 Department of Stomatology, Faculty of Dentistry of Bauru;
2 Department of Pharmacology and
3 Biochemistry and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Brazil;
4 Department of Basic Sciences, School of Dentistry, Araçatuba State University of São Paulo; and
5 Department of Basic Sciences, University of Texas-Houston Health Science Center Dental Branch, Houston, TX, USA;

Correspondence: * corresponding author, tarcilia{at}zipmail.com.br.

Dentin sialoprotein (DSP) and dentin phosphoprotein (DPP), the major dentin proteins, have been shown to induce neutrophil migration through release of IL-1β, TNF-{alpha}, MIP-2, and KC. However, the sources of these mediators were not determined. Here, the roles of macrophages and mast cells (MC) in dentin-induced neutrophil accumulation were investigated. Peritoneal MC depletion or the enhancement of macrophage population increased DSP- and DPP-induced neutrophil extravasation. Moreover, supernatants from DSP- and DPP-stimulated macrophages caused neutrophil migration. The release of neutrophil chemotactic factor by macrophages was inhibited by dexamethasone or the supernatant of DSP-treated MC. Consistently, dexamethasone and the MC supernatant inhibited the production of IL-1β, TNF-{alpha}, and MIP-2 by macrophages. This inhibitory activity of the DSP-stimulated MC was neutralized by anti-IL-4 and anti-IL-10 antibodies. These results indicate that dentin induces the release of the neutrophil chemotactic substance(s) by macrophages, which are down-modulated by MC-derived IL-4 and IL-10.

Key Words: neutrophil chemotaxis • DSP • DPP

Journal of Dental Research, Vol. 84, No. 1, 79-83 (2005)
DOI: 10.1177/154405910508400114
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