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Journal of Dental Research
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Biological

Cyclic Mechanical Strain Regulates the PTHrP Expression in Cultured Chondrocytes via Activation of the Ca2+ Channel

N. Tanaka1,§, S. Ohno1,*,§, K. Honda1, K. Tanimoto1, T. Doi1, M. Ohno-Nakahara1, E. Tafolla2, S. Kapila3 and K. Tanne1

1 Departments of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi, Minami-Ku, Hiroshima 734-8553, Japan;
2 Department of Stomatology, University of California-San Francisco, San Francisco, CA, USA; and
3 Department of Orthodontics and Pediatric Dentistry, School of Dentistry, University of Michigan, Ann Arbor, USA;

Correspondence: * corresponding author, shigebon{at}hiroshima-u.ac.jp

The association between mechanical stimulation and chondrocyte homeostasis has been reported. However, the participation of PTHrP (parathyroid-hormone-related protein) in the mechano-regulation of chondrocyte metabolism remains unclear. We determined whether mechanical stimulation of chondrocytes induces the expression of PTHrP and, further, whether the mechano-modulation of PTHrP is dependent on the maturational status of chondrocytes. Cyclic mechanical strain was applied to rat growth plate chondrocytes at the proliferating, matrix-forming, and hypertrophic stages at 30 cycles/min. Cyclic mechanical strain significantly increased PTHrP mRNA levels in chondrocytes at the proliferating and matrix-forming stages only. The induction of PTHrP was dependent on loading magnitude at the proliferating stage. Using specific ion channel blockers, we determined that mechano-induction of PTHrP was inhibited by nifedipine, a Ca2+ channel blocker. These results suggest that mechanical induction of PTHrP possibly provides the environment for greater chondrocyte replication and matrix formation that would subsequently affect cartilage formation.

Key Words: mechanical strain • chondrocytes • PTHrP • ion channels

Journal of Dental Research, Vol. 84, No. 1, 64-68 (2005)
DOI: 10.1177/154405910508400111
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