This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Kawazoe, Y. Articles by Kohgo, T. Search for Related Content PubMed PubMed Citation Articles by Kawazoe, Y. Articles by Kohgo, T. Pubmed/NCBI databases Substance via MeSH Social Bookmarking                         What's this?

Induction of Calcification in MC3T3-E1 Cells by Inorganic Polyphosphate

Y. Kawazoe^1,2,3, T. Shiba^1,2,5,*, R. Nakamura⁴, A. Mizuno², K. Tsutsumi^4,, T. Uematsu⁵, M. Yamaoka⁵, M. Shindoh³ and T. Kohgo³

¹ Regenetiss Co., Ltd., 1-5-17, Akabane, Okaya, Nagano 394-0002, Japan;
² Frontier Research Division, Fujirebio Inc., Hachioji, Tokyo, Japan;
³ Graduate School of Dental Medicine, and
⁴ Graduate School of Engineering, Hokkaido University, Sapporo, Hokkaido, Japan; and
⁵ Matsumoto Dental University, Shiojiri, Nagano, Japan;

Correspondence: ^* corresponding author, shiba{at}regenetiss.com

Relatively large amounts of inorganic polyphosphate [poly(P)] (400 µM) have been found in normal osteoblasts. The effect of poly(P) with an average chain length of 65 phosphate residues on cell calcification was therefore investigated with the use of MC3T3-E1 cells. Expression of both osteopontin and osteocalcin was induced by poly(P) (0.1 ~ 1 mM), and cells treated with poly(P) were strongly stained by alizarin red. In addition, the level of alkaline phosphatase activity induced in poly(P)-treated cells was two-fold higher than that in either orthophosphate-treated or control cells but not higher than that in cells treated with β-glycerophosphate and ascorbic acid. In contrast, however, polyphosphatase activities were activated by poly(P) treatment to levels up to six-fold greater than that in controls. MC3T3-E1 cells may utilize poly(P) as a phosphate source for calcification rather than phosphate sources that are mainly produced by ALPase. Poly(P)-dependent induction of polyphosphatase activities may therefore promote calcification in MC3T3-E1 cells.

Key Words: calcification • osteoblasts • osteopontin • osteocalcin • polyphosphate

Journal of Dental Research, Vol. 83, No. 8, 613-618 (2004)
DOI: 10.1177/154405910408300806


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				S. A. Smith and J. H. Morrissey Polyphosphate enhances fibrin clot structure Blood, October 1, 2008; 112(7): 2810 - 2816. [Abstract] [Full Text] [PDF]

				S. Holbein, F. M. Freimoser, T. P. Werner, A. Wengi, and B. Dichtl Cordycepin-hypersensitive growth links elevated polyphosphate levels to inhibition of poly(A) polymerase in Saccharomyces cerevisiae Nucleic Acids Res., February 2, 2008; 36(2): 353 - 363. [Abstract] [Full Text] [PDF]

				W. N. Addison, F. Azari, E. S. Sorensen, M. T. Kaartinen, and M. D. McKee Pyrophosphate Inhibits Mineralization of Osteoblast Cultures by Binding to Mineral, Up-regulating Osteopontin, and Inhibiting Alkaline Phosphatase Activity J. Biol. Chem., May 25, 2007; 282(21): 15872 - 15883. [Abstract] [Full Text] [PDF]