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Journal of Dental Research
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Biological

Cementoblast Gene Expression is Regulated by Porphyromonas gingivalis Lipopolysaccharide Partially via Toll-like Receptor-4/MD-2

F.H. Nociti, Jr.1,2, B.L. Foster2, S.P. Barros3, R.P. Darveau2 and M.J. Somerman2,*

1 Department of Prosthodontics/Periodontics, Division of Periodontics, School of Dentistry at Piracicaba, University of Campinas, Brazil;
2 Department of Periodontics, School of Dentistry, 1959 NE Pacific, D322-Health Science Center, University of Washington, Seattle, WA 98195-7444, USA; and
3 Department of Morphology, Division of Histology, School of Dentistry at Piracicaba, University of Campinas, Brazil;

Correspondence: * corresponding author, somerman{at}u.washington.edu

Lipopolysaccharides are potent inflammatory mediators considered to contribute to destruction of periodontal tissues. Here, we hypothesized that Porphyromonas gingivalis lipopolysaccharide (P-LPS) treatment would regulate gene expression in murine cementoblasts through Toll-like receptor 4. Real-time (RT)-PCR and Northern blot analysis indicated that P-LPS decreased expression of transcripts for osteocalcin (OCN) and receptor activator of nuclear factor {kappa}B ligand (RANKL). In contrast, a dose-dependent up-regulation in mRNA levels for osteopontin (OPN) and osteoprotegerin (OPG) was observed. Similarly, ELISA demonstrated decreased RANKL and increased OPG levels. A monoclonal antibody specific for mouse TLR-4/MD-2 partially neutralized the P-LPS effect on cementoblasts. These results indicate that exposure of cementoblasts to P-LPS can alter cell function by regulating markers of osteoclastic activity (e.g., RANKL/OPG), thereby potentially affecting the inflammation-associated resorption of mineralized tissues.

Key Words: lipopolysaccharides • cementoblasts • cytokines • Porphyromonas gingivalis

Journal of Dental Research, Vol. 83, No. 8, 602-607 (2004)
DOI: 10.1177/154405910408300804
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