Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Danciu, T.E.
Right arrow Articles by Freeman, M.R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Danciu, T.E.
Right arrow Articles by Freeman, M.R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Biological

Mechanical Strain Delivers Anti-apoptotic and Proliferative Signals to Gingival Fibroblasts

T.E. Danciu1, E. Gagari2, R.M. Adam1, P.D. Damoulis2 and M.R. Freeman1

1 Enders Research Laboratories, Rm 1150.2, Children’s Hospital, 300 Longwood Avenue, Boston, MA 02115, USA, and Department of Surgery, Harvard Medical School, Boston; and
2 Tufts School of Dental Medicine, Boston, MA, USA;

Correspondence: * corresponding author, Theodora.Danciu{at}tch.harvard.edu

Physical forces play a critical role in the survival and proliferation of many cell types, including fibroblasts. Gingival fibroblasts are exposed to mechanical stress during mastication, orthodontic tooth movement, and wound healing following periodontal surgery. The aim of this study was to examine the effect of mechanical strain on human gingival fibroblasts (hGF). Cells were subjected to short-term (up to 60 min) and long-term (up to 48 hrs) 20% average elongation at 0.1 Hz. We monitored survival signaling by evaluating the phosphorylation status and localization of Forkhead box (FoxO) family members, which are mediators of apoptosis. We also examined strain-induced proliferation by measuring the level of proliferating cell nuclear antigen (PCNA). We observed that cyclic strain caused the phosphorylation and retention in the cytoplasm of FoxO family members. Moreover, mechanical strain resulted in increased ERK kinase phosphorylation and PCNA expression. In conclusion, cyclic strain delivers anti-apoptotic and proliferative stimuli to hGF.

Key Words: gingival fibroblasts • stretch • FoxO • MAPK • PCNA

Journal of Dental Research, Vol. 83, No. 8, 596-601 (2004)
DOI: 10.1177/154405910408300803
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 JDRHome page
V. Krishnan and Z. Davidovitch
On a Path to Unfolding the Biological Mechanisms of Orthodontic Tooth Movement
Journal of Dental Research, July 1, 2009; 88(7): 597 - 608.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
P. S. Pardo, M. A. Lopez, and A. M. Boriek
FOXO transcription factors are mechanosensitive and their regulation is altered with aging in the respiratory pump
Am J Physiol Cell Physiol, April 1, 2008; 294(4): C1056 - C1066.
[Abstract] [Full Text] [PDF]