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Journal of Dental Research
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Biological

Monocyte Activation by Porphyromonas gingivalis LPS in Aggressive Periodontitis with the Use of Whole-blood Cultures

R. Mahanonda1,2,*, N. Sa-Ard-Iam2, O. Charatkulangkun1, A. Promsudthi3, R.E. Schifferle4, K. Yongvanichit5 and S. Pichyangkul5

1 Department of Periodontology, Faculty of Dentistry, Chulalongkorn University, Henry Dunant Rd., Bangkok 10330, Thailand;
2 Immunology Lab, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand;
3 Department of Oral Medicine, Faculty of Dentistry, Mahidol University, Bangkok, Thailand;
4 Department of Periodontics & Endodontics, State University of New York at Buffalo, Buffalo, NY, USA;
5 Department of Immunology and Medicine, US Army Medical Component, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, Thailand;

Correspondence: * corresponding author, mrangsin{at}chula.ac.th

In this study, we re-visited the issue of hyper-responsiveness of monocytes to bacterial lipopolysaccharide (LPS) in aggressive periodontitis patients. We used whole-blood cultures to compare monocyte activation by Porphyromonas gingivalis LPS between Thai subjects with generalized aggressive periodontitis and those without periodontitis. Upon stimulation with P. gingivalis LPS, expression of co-stimulatory molecules on monocytes and expression of CD69 on NK and {gamma}{delta} T-cells were analyzed by flow cytometry, and the production of interleukin-1β and prostaglandin E2 was monitored by ELISA. LPS stimulation resulted in a dose-dependent up-regulation of CD40, CD80, and CD86 on monocytes, and up-regulation of CD69 on NK cells and {gamma}{delta} T-cells in both the periodontitis and non-periodontitis groups. The levels of activation markers and the mediator production after LPS stimulation were quite similar for both groups. In conclusion, we did not observe hyper-responsiveness of monocytes to P. gingivalis LPS challenge in Thai patients with aggressive periodontitis.

Key Words: monocyte activation • Porphyromonas gingivalis • co-stimulatory molecule expression • whole-blood cultures

Journal of Dental Research, Vol. 83, No. 7, 540-545 (2004)
DOI: 10.1177/154405910408300706
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