This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Sato, Y. Articles by Kizaki, H. Search for Related Content PubMed PubMed Citation Articles by Sato, Y. Articles by Kizaki, H. Pubmed/NCBI databases Protein Substance via MeSH Social Bookmarking                         What's this?

Streptococcus mutans Strains Harboring Collagen-binding Adhesin

¹ Department of Biochemistry and
² Oral Health Science Center, Tokyo Dental College, 2-2, Masago 1-chome, Mihama-ku, Chiba City, 261-8502 Japan; and
³ Department of Oral Microbiology, Showa University School of Dentistry, Japan;

Correspondence: ^* corresponding author, yusato{at}tdc.ac.jp

A previously unidentified 120-kDa protein was detected in Streptococcus mutans strain Z1 and was involved in the cold-agglutination of the strain. We have identified the gene, designated cnm, as being involved in the agglutination of strain Z1 following random mutagenesis. The amino acid sequence of the deduced Cnm protein exhibited high similarity to those of collagen-binding adhesins from staphylococci and other organisms. To confirm whether the protein is involved in collagen-binding, we cloned a cnm gene fragment, overexpressed it in E.coli, and prepared crude extracts. The extracts containing recombinant protein exhibited binding to immobilized collagen and laminin but not to fibronectin. Compared with the parental strain Z1, the cold-agglutination-negative mutant 05A02 exhibited reduced binding to collagen and laminin but retained that to fibronectin. This gene was detected in some strains of S. mutans. Therefore, the cnm gene encoded a new strain-specific member of the collagen-binding adhesin family.

Key Words: Streptococcus mutans • collagen-binding adhesin • streptococcal endocarditis • strain-specific gene

Journal of Dental Research, Vol. 83, No. 7, 534-539 (2004)
DOI: 10.1177/154405910408300705


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				A. H. Nobbs, R. J. Lamont, and H. F. Jenkinson Streptococcus Adherence and Colonization Microbiol. Mol. Biol. Rev., September 1, 2009; 73(3): 407 - 450. [Abstract] [Full Text] [PDF]

				R. Nomura, K. Nakano, N. Taniguchi, J. Lapirattanakul, H. Nemoto, L. Gronroos, S. Alaluusua, and T. Ooshima Molecular and clinical analyses of the gene encoding the collagen-binding adhesin of Streptococcus mutans J. Med. Microbiol., April 1, 2009; 58(4): 469 - 475. [Abstract] [Full Text] [PDF]

				K. Nakano, J. Lapirattanakul, R. Nomura, H. Nemoto, S. Alaluusua, L. Gronroos, M. Vaara, S. Hamada, T. Ooshima, and I. Nakagawa Streptococcus mutans Clonal Variation Revealed by Multilocus Sequence Typing J. Clin. Microbiol., August 1, 2007; 45(8): 2616 - 2625. [Abstract] [Full Text] [PDF]

				S. R. Nallapareddy, J. Sillanpaa, V. K. Ganesh, M. Hook, and B. E. Murray Inhibition of Enterococcus faecium Adherence to Collagen by Antibodies against High-Affinity Binding Subdomains of Acm Infect. Immun., June 1, 2007; 75(6): 3192 - 3196. [Abstract] [Full Text] [PDF]

				K. Nakano, H. Nemoto, R. Nomura, H. Homma, H. Yoshioka, Y. Shudo, H. Hata, K. Toda, K. Taniguchi, A. Amano, et al. Serotype distribution of Streptococcus mutans a pathogen of dental caries in cardiovascular specimens from Japanese patients J. Med. Microbiol., April 1, 2007; 56(4): 551 - 556. [Abstract] [Full Text] [PDF]

				J. C. Waterhouse and R. R. B. Russell Dispensable genes and foreign DNA in Streptococcus mutans Microbiology, June 1, 2006; 152(6): 1777 - 1788. [Abstract] [Full Text] [PDF]