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Journal of Dental Research
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Biological

Construction and Immunogenic Characterization of a Fusion Anti-caries DNA Vaccine against PAc and Glucosyltransferase I of Streptococcus mutans

J.H. Guo{dagger}, R. Jia{dagger}, M.W. Fan*, Z. Bian, Z. Chen and B. Peng

Key Lab. for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, PR China;

Correspondence: * corresponding author, kqyywjtx{at}public.wh.hb.cn

Glucosyltransferases (GTFs) and A cell-surface protein (PAc) are two important virulence factors of the cariogenic organism Streptococcus mutans. They may mediate sucrose-independent or sucrose-dependent attachment of Streptococcus mutans to tooth surfaces, respectively. Thus, inhibiting both virulence factors is predicted to provide better protection against caries than inhibiting a single factor. To develop a highly efficient vaccine against caries, we constructed a fusion DNA vaccine, pGLUA-P, by cloning the GLU region of GTF into a DNA vaccine, pCIA-P, which encodes two highly conservative regions of PAc. In this report, we provide evidence that fewer caries lesions were observed in rats following subcutaneous injection of pGLUA-P, compared with pCIA-P, near the submandibular gland. Our findings suggest that a multigenic DNA vaccine may be more caries-preventive than a single-gene DNA vaccine.

Key Words: Streptococcus mutans • PAc • glucosyltransferase • DNA vaccine • dental caries

Journal of Dental Research, Vol. 83, No. 3, 266-270 (2004)
DOI: 10.1177/154405910408300316
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