Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Kamolmatyakul, S.
Right arrow Articles by Li, Y.-P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kamolmatyakul, S.
Right arrow Articles by Li, Y.-P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Biological

IL-1{alpha} Stimulates Cathepsin K Expression in Osteoclasts via the Tyrosine Kinase-NF-{kappa}B Pathway

S. Kamolmatyakul1, W. Chen1,2, S. Yang1,2, Y. Abe1, R. Moroi1, A.M. Ashique1,2 and Y.-P. Li1,2,*

1 Department of Cytokine Biology, The Forsyth Institute, 140 The Fenway, Boston, MA 02115, USA; and 2 Harvard-Forsyth Department of Oral Biology, The Forsyth Institute & Harvard School of Dental Medicine, Boston, MA 02115, USA;

Correspondence: * corresponding author, ypli{at}forsyth.org

Interleukin-1{alpha} (IL-1{alpha}) is a powerful activator of osteoclast cells. However, the underlying mechanism for this activation is unknown. In this study, we reveal that IL-1{alpha} up-regulates the expression of cathepsin K protein, a key protease in bone resorption, by five-fold. Northern blot analysis and promoter analysis show that this induction occurs at the transcriptional level, in a dose-responsive and time-dependent manner. No increase in expression occurs in the presence of either pyrrolidine dithiocarbamate (PDTC), a selective inhibitor of NF-{kappa}B, or Genistein, a protein tyrosine kinase inhibitor, suggesting that IL-1{alpha} up-regulation may be via the tyrosine kinase-NF-{kappa}B pathway to regulate cathepsin K expression. Antisense oligonucleotides to p65, but not the p50 subunit of NF-{kappa}B, suppress the IL-1{alpha}-induced expression of cathepsin K. We therefore conclude that IL-1{alpha} up-regulates cathepsin K gene expression at the transcription level, and this regulation may be via the tyrosine-kinase-NF-{kappa}B pathway.

Key Words: interleukin-1alpha • cathepsin K • osteoclast • NF-{kappa}B • tyrosine kinase

Journal of Dental Research, Vol. 83, No. 10, 791-796 (2004)
DOI: 10.1177/154405910408301011
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Eur J OrthodHome page
V. Vandevska-Radunovic and R. Murison
Emotional stress and orthodontic tooth movement: effects on apical root resorption, tooth movement, and dental tissue expression of interleukin-1 alpha and calcitonin gene-related peptide immunoreactive nerve fibres in rats
Eur J Orthod, November 16, 2009; (2009) cjp106v1.
[Abstract] [Full Text] [PDF]