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Osteoblast Precursor Cell Attachment on Heat-treated Calcium Phosphate Coatings

¹ University of Texas Health Science Center at San Antonio, Department of Restorative Dentistry, Division of Biomaterials, MSC 7890, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900;
² Mississippi State University, Department of Agricultural and Biological Engineering, Box 9632, Mississippi State, MS 39762;
³ University of Texas Health Science Center at San Antonio, Department of Periodontics, MSC 7894, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900; and
⁴ University of Texas Health Science Center at San Antonio, Center for Clinical Bioengineering, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900;

Correspondence: *corresponding author, Ong{at}uthscsa.edu

The influence of properties of calcium phosphate (CaP) coatings on bone cell activity and bone-implant osseointegration is not well-established. This study investigated the effects of characterized CaP coatings of various heat treatments on osteoblast response. It was hypothesized that heat treatments of CaP coatings alter the initial osteoblast attachment. The 400°C heat-treated coatings were observed to exhibit poor crystallinity and significantly greater phosphate or apatite species compared with as-sputtered and 600°C heat-treated coatings. Similarly, human embryonic palatal mesenchyme (HEPM) cells, an osteoblast precursor cell line, seeded on 400°C heat-treated coatings, exhibited significantly greater cell attachment compared with Ti surfaces, as-sputtered coatings, and 600°C heat-treated coatings. The HEPM cells on Ti surfaces and heat-treated coatings were observed to attach through filopodia, and underwent cell division, whereas the cells on as-sputtered coatings displayed fewer filopodia extensions and cell damage. Analysis of the data suggested that heat treatment of CaP coatings affects cell attachment.

Key Words: calcium phosphate coating • titanium • osteobalst • attachment • heat treatment

Journal of Dental Research, Vol. 82, No. 6, 449-453 (2003)
DOI: 10.1177/154405910308200609


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