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Journal of Dental Research
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Biological

Regulation of Osteoprotegerin Gene Expression in Dental Follicle Cells

G.E. Wise*, Y. Ren and S. Yao

Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803;

Correspondence: *corresponding author, gwise{at}vetmed.lsu.edu

Colony-stimulating factor-one (CSF-1) and parathyroid-hormone-related protein (PTHrP) down-regulate osteoprotegerin (OPG) gene expression in the dental follicle of the rat first mandibular molar. To examine this regulation at the signal transduction level, we treated cultured dental follicle cells with either phorbolmyristate acetate (PMA) or dibutyryl cyclic AMP (dbcAMP) to activate either protein kinase C (PKC) or protein kinase A (PKA). Our results demonstrate that PMA up-regulates OPG gene expression and down-regulates the expression of CSF-1 and the PTHrP receptor (PTHrP-R). Conversely, dbcAMP down-regulates OPG expression and up-regulates CSF-1 and PTHrP-R expression. Immunostaining shows that PMA also increases the steady-state levels of protein. Thus, treatment with agents that affect protein kinase activity also enhance the steady-state mRNA and protein levels of OPG, as well as decreasing the mRNA levels of CSF-1 and PTHrP-R. The PKC-{alpha} isoform may be critical in OPG regulation because PKC-{alpha} gene expression is enhanced by PMA and reduced by either CSF-1 or PTHrP.

Key Words: dental follicle • PKC • PKA • osteoprotegerin • tooth eruption

Journal of Dental Research, Vol. 82, No. 4, 298-302 (2003)
DOI: 10.1177/154405910308200411
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