This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Krisanaprakornkit, S. Articles by Dale, B.A. Search for Related Content PubMed PubMed Citation Articles by Krisanaprakornkit, S. Articles by Dale, B.A. Social Bookmarking                         What's this?

Intracellular Calcium in Signaling Human β-Defensin-2 Expression in Oral Epithelial Cells

¹ Department of Odontology-Oral Pathology,
² Department of Orthodontics, Faculty of Dentistry, Chiang Mai University, Chiang Mai 50200, Thailand; and
³ Department of Oral Biology, School of Dentistry, University of Washington, Seattle, WA 98195, USA;

Correspondence: ^* corresponding author, sutichai{at}chiangmai.ac.th, suttichaikris{at}yahoo.com

Expression of human β-defensins is correlated with differentiation in the oral epithelium, consistent with their function as part of the epithelial antimicrobial barrier. Because calcium is a known regulator of epithelial differentiation, we tested the hypothesis that calcium concentration mediates β-defensin expression. Gingival epithelial cells were cultured in medium containing low calcium concentration (0.03 mM), then either changed to high extracellular calcium concentrations or stimulated with thapsigargin to release intracellular calcium stores in the presence or absence of BAPTA-AM, a calcium chelator. Human β-defensin-2 (hBD-2) mRNA expression was rapidly induced by thapsigargin, and more slowly induced by high extracellular calcium. Induction of hBD-2 peptide was confirmed by immunofluorescence. BAPTA-AM inhibited hBD-2 induction by both thapsigargin and calcium in a dose-dependent fashion. In addition, BAPTA-AM inhibited hBD-2 induction by a bacterial stimulant. Collectively, these findings demonstrate that intracellular calcium is a critical mediator of hBD-2 expression. Abbreviations used in this study are: BAPTA-AM, 1,2-bis(2-aminophenoxy)-ethane-N,N,N',N'-tetra-acetic acid tetrakis (acetoxymethyl ester); DMSO, dimethylsulfoxide; F. nucleatum, Fusobacterium nucleatum; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HBDs, human β-defensins; HGECs, human gingival epithelial cells; MAP, mitogen-activated protein; and RT-PCR, reverse-transcriptase/polymerase chain-reaction.

Key Words: innate immunity • antimicrobial peptide • β-defensins • calcium • thapsigargin

Journal of Dental Research, Vol. 82, No. 11, 877-882 (2003)
DOI: 10.1177/154405910308201106


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				P. Montreekachon, P. Chotjumlong, V. Reutrakul, and S. Krisanaprakornkit Involvement of Cytosolic Phospholipase A2{alpha} in MMP-9 Up-regulation Journal of Dental Research, November 1, 2009; 88(11): 1031 - 1036. [Abstract] [Full Text] [PDF]

				S. Krisanaprakornkit, P. Chotjumlong, P. Kongtawelert, and V. Reutrakul Involvement of phospholipase D in regulating expression of anti-microbial peptide human -defensin-2 Int. Immunol., January 1, 2008; 20(1): 21 - 29. [Abstract] [Full Text] [PDF]

				H. Dommisch, W. O. Chung, M. G. Rohani, D. Williams, M. Rangarajan, M. A. Curtis, and B. A. Dale Protease-Activated Receptor 2 Mediates Human Beta-Defensin 2 and CC Chemokine Ligand 20 mRNA Expression in Response to Proteases Secreted by Porphyromonas gingivalis Infect. Immun., September 1, 2007; 75(9): 4326 - 4333. [Abstract] [Full Text] [PDF]

				B. E. Menzies and A. Kenoyer Staphylococcus aureus Infection of Epidermal Keratinocytes Promotes Expression of Innate Antimicrobial Peptides Infect. Immun., August 1, 2005; 73(8): 5241 - 5244. [Abstract] [Full Text] [PDF]

				W. O. Chung, S. R. Hansen, D. Rao, and B. A. Dale Protease-Activated Receptor Signaling Increases Epithelial Antimicrobial Peptide Expression J. Immunol., October 15, 2004; 173(8): 5165 - 5170. [Abstract] [Full Text] [PDF]