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Journal of Dental Research
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Biomaterials & Bioengineering

TEGDMA Causes Apoptosis in Primary Human Gingival Fibroblasts

V. Janke1,*, N. von Neuhoff2,*, B. Schlegelberger2, G. Leyhausen1 and W. Geurtsen3,+

1 Department of Conservative Dentistry & Periodontology
2 Institute of Cell and Molecular Pathology, Medical Univ. Hannover, Germany; and
3 Department of Restorative Dentistry, School of Dentistry, University of Washington, Box 357456, Seattle, WA 98195-7456, USA;

Correspondence: +corresponding author, wgert{at}u.washington.edu

Previous in vivo studies have revealed that resins may generate a persistent inflammation of oral tissues and cell death as well. Apoptosis is an important regulated process that results in rapid cell death. This study tested the hypothesis that the comonomer triethyleneglycol-dimethacrylate (TEGDMA) causes apoptosis. The effects of TEGDMA on proliferation and apoptosis in primary oral fibroblasts were analyzed by light microscopy and flow cytometry (FACS; Annexin V-assay). TEGDMA at 5 and 7.5 mM inhibited proliferation after 24 hrs. No increased frequency of apoptosis or necrosis was observed with 1 mM or 2.5 mM TEGDMA after 24 hrs. Apoptosis and Annexin V-positive cells were observed with 5 mM and 7.5 mM TEGDMA by light microscopy after 24 hrs. A dramatic increase in apoptotic cells was detected by FACS after 24 hrs with 7.5 mM TEGDMA. Thus, TEGDMA was cytotoxic and "apoptotic" in a dose- and time-dependent manner.

Key Words: TEGDMA • apoptosis • necrosis • gingival fibroblasts

Journal of Dental Research, Vol. 82, No. 10, 814-818 (2003)
DOI: 10.1177/154405910308201010
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