This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Gemmell, E. Articles by Seymour, G.J. Search for Related Content PubMed PubMed Citation Articles by Gemmell, E. Articles by Seymour, G.J. Pubmed/NCBI databases Substance via MeSH Social Bookmarking                         What's this?

P. gingivalis-specific T-cell Lines Produce Th1 and Th2 Cytokines

Immunopathology Laboratory, Oral Biology and Pathology, School of Dentistry, The University of Queensland, Brisbane 4072, Australia;

Correspondence: *corresponding author, e.gemmell{at}mailbox.uq.edu.au

Cytokines produced by T-cells in periodontal lesions may determine the nature of the adaptive immune response. Since different antigen-presenting cells (APC) may direct the Th1/Th2 response, P. gingivalis-specific T-cell lines were established by different APC subpopulations, and their cytokine profiles were determined. Peripheral blood mononuclear cells induced similar percentages of IL-4+ and IFN-gamma+ T-cells and lower percentages of IL-10+ T-cells. Epstein-Barr virus-transformed B-cells (LCL) induced higher percentages of IL-4+ cells than IFN-gamma+ cells, with lower percentages of IL-10+ cells. Peripheral blood mononuclear cells induced a higher percent of IFN-gamma+ CD8 cells than LCL (p = 0.004). Purified B-cells, monocytes, and dendritic cells induced similar percentages of IL-4+ and IFN-gamma+ cells, although again, the percentage of IL-10+ cells was lower. The results of the present study have demonstrated that, as measured by FACS analysis of intracytoplasmic cytokines, P. gingivalis-specific T-cells produce both Th1 and Th2 cytokines, regardless of the APC population.

Key Words: P. gingivalis • T-cells • periodontal disease • cytokines • antigen-presenting cells

Journal of Dental Research, Vol. 81, No. 5, 303-307 (2002)
DOI: 10.1177/154405910208100503


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				C. W. Leone, H. Bokhadhoor, D. Kuo, T. Desta, J. Yang, M. F. Siqueira, S. Amar, and D. T. Graves Immunization Enhances Inflammation and Tissue Destruction in Response to Porphyromonas gingivalis Infect. Immun., April 1, 2006; 74(4): 2286 - 2292. [Abstract] [Full Text] [PDF]

				P. Ford, E. Gemmell, P. Walker, M. West, M. Cullinan, and G. Seymour Characterization of Heat Shock Protein-Specific T Cells in Atherosclerosis Clin. Vaccine Immunol., February 1, 2005; 12(2): 259 - 267. [Abstract] [Full Text] [PDF]

				F. Goulhen, D. Grenier, and D. Mayrand ORAL MICROBIAL HEAT-SHOCK PROTEINS AND THEIR POTENTIAL CONTRIBUTIONS TO INFECTIONS Critical Reviews in Oral Biology & Medicine, November 1, 2003; 14(6): 399 - 412. [Abstract] [Full Text] [PDF]

				Y.-T. A. Teng THE ROLE OF ACQUIRED IMMUNITY AND PERIODONTAL DISEASE PROGRESSION Critical Reviews in Oral Biology & Medicine, July 1, 2003; 14(4): 237 - 252. [Abstract] [Full Text] [PDF]