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Journal of Dental Research
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Biological

RGD-CAP (βig-h3) Exerts a Negative Regulatory Function on Mineralization in the Human Periodontal Ligament

S. Ohno1,*, T. Doi1, K. Fujimoto2, C. Ijuin1, N. Tanaka1, K. Tanimoto1, K. Honda1, M. Nakahara1, Y. Kato2 and K. Tanne1

1 Departments of Orthodontics and
2 Biochemistry, Hiroshima University Faculty of Dentistry, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan;

Correspondence: *corresponding author, shigebon{at}hiroshima-u.ac.jp

In our previous studies, RGD-CAP/βig-h3 was isolated from a fiber-rich fraction of cartilage and was found to have a negative function on mineralization of hypertrophic chondrocytes. However, the expression and biological function of RGD-CAP in the periodontal ligament (PDL) are not known. We hypothesized that RGD-CAP could be expressed in the PDL and regulate its mineralization. To test this, we investigated the expression of RGD-CAP in human PDL and the effects of RGD-CAP on mineralization of cultured PDL cells. RGD-CAP was detected in the human PDL as multimeric proteins greater than 200 kDa. The RGD-CAP mRNA level decreased in cultured PDL cells exposed to 10–8 M dexamethasone or 10–8 M 1{alpha},25-dihydroxyvitamin D3 when these steroids increased alkaline phosphatase (ALP) activity. Furthermore, exogenous RGD-CAP suppressed the ALP activity and bone nodule formation of cultured PDL cells. These findings suggest that RGD-CAP in the PDL modulates the mineralization which affects adjacent alveolar bone metabolism.

Key Words: periodontal ligament • RGD-CAP/βig-h3 • alkaline phosphatase • mineralization

Journal of Dental Research, Vol. 81, No. 12, 822-825 (2002)
DOI: 10.1177/154405910208101205
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