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Journal of Dental Research
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Biological

Staurosporine Mobilizes Ca2+ from Secretory Granules by Inhibiting Protein Kinase C in Rat Submandibular Acinar Cells

Y.J. Kim1,*, J.M. An1,*, D.M. Shin1, S.-I. Lee1, H. Sugiya2 and J.T. Seo1,3

1 Department of Oral Biology & Oral Science Research Center, BK21 Project for Medical Sciences, Yonsei University College of Dentistry, Shinchon-dong 134, Seodaemoon-gu, Seoul 120-752, Korea; and
2 Department of Physiology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan;

Correspondence: 3 corresponding author, jeong{at}yumc.yonsei.ac.kr

Staurosporine was previously shown to mobilize Ca2+ from the thapsigargin-insensitive Ca2+ store in rat submandibular acinar cells. However, the nature of the store is not yet known. Therefore, in the present study, the staurosporine-releasable intracellular Ca2+ store was characterized. Staurosporine increased the cytosolic Ca2+ concentration ([Ca2+]c) after the inositol 1,4,5-trisphosphate (IP3)-sensitive Ca2+ store was depleted. Ionomycin caused only small increases in [Ca2+]c after the depletion of the IP3-sensitive Ca2+ store, whereas ionomycin+monensin caused large increases. However, ionomycin+monensin did not increase [Ca2+]c when added after [Ca2+]c was increased by staurosporine, indicating that the acidic Ca2+ store was the main source of Ca2+. The acidic Ca2+ store appeared to be associated with secretory granules, since ionomycin+monensin- and staurosporine-induced [Ca2+]c increases were significantly reduced when the acinar cells were degranulated. The effect of staurosporine on [Ca2+]c was mimicked by other protein kinase C inhibitors. Therefore, we conclude that staurosporine mobilizes Ca2+ from secretory granules, probably through the inhibition of protein kinase C in rat submandibular acinar cells.

Key Words: Secretory granules • staurosporine • submandibular acinar cells • calcium

Journal of Dental Research, Vol. 81, No. 11, 788-793 (2002)
DOI: 10.1177/154405910208101113
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