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Journal of Dental Research
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Biological

Regulation of TNF-{alpha}-induced IL-6 Production in MG-63 Human Osteoblast-like Cells

S.J. Webb1, J.R. McPherson2, K. Pahan3 and S. Koka*,3

1 Formerly Dept. of Oral Biology, UNMC, and presently Dept. of Orthodontics, The Ohio State University, Columbus, OH;
2 formerly Dept. of Oral Biology, UNMC, and presently in private practice, Roseburg, OR;
3 Dept. of Oral Biology, UNMC, 40th & Holdrege Sts., Lincoln, NE 68583;

Correspondence: *corresponding author, skoka{at}unmc.edu

Tumor necrosis factor-{alpha} (TNF-{alpha}) stimulates osteoblast production of interleukin-6 (IL-6), an inflammatory cytokine implicated in osteoclastic bone resorption. Therefore, we tested the hypothesis that TNF-{alpha}-induced IL-6 production in MG-63 osteosarcoma cells occurs via the p38 mitogen-activated protein kinase (MAPK) pathway. TNF-{alpha} activated p38 MAPK and stimulated IL-6 secretion by MG-63 cells, and pre-incubation of cells with the p38 MAPK inhibitor abrogated TNF-{alpha}-dependent IL-6 secretion. Transfection of IL-6 full-length and 5'-deletion gene promoter reporter constructs indicated that p38 MAPK activation by TNF-{alpha} enhanced IL-6 gene expression, and that the p38 MAPK-responsive region resided in the proximal 260-bp segment. Transfection of NF{kappa}B and C/EBPβ-sensitive reporter promoter constructs demonstrated that NF{kappa}B activity was enhanced and that constitutive C/EBPβ was inhibited by TNF-{alpha}, with both effects being p38 MAPK-dependent. In conclusion, although p38 MAPK activation by TNF-{alpha} stimulates IL-6 secretion by MG-63 cells, it has opposing effects on c/EBPβ and NF{kappa}B activity.

Key Words: map kinase • interleukin-6 • osteoblast • inflammation

Journal of Dental Research, Vol. 81, No. 1, 17-22 (2002)
DOI: 10.1177/154405910208100105
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