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Effects of Dental Resins on TNF--induced ICAM-1 Expression in Endothelial Cells

University of Missouri-Kansas City, Schools of Pharmacy and Dentistry, 2411 Holmes Street, M4-209, Kansas City, MO 64108, USA

P.Y. Tong

University of Missouri-Kansas City, Schools of Pharmacy and Dentistry, 2411 Holmes Street, M4-209, Kansas City, MO 64108, USA

A.F. Strautman

William Jewell College, Liberty, MO, USA

A.G. Glaros

University of Missouri-Kansas City, Schools of Pharmacy and Dentistry, 2411 Holmes Street, M4-209, Kansas City, MO 64108, USA

J.D. Eick

University of Missouri-Kansas City, Schools of Pharmacy and Dentistry, 2411 Holmes Street, M4-209, Kansas City, MO 64108, USA

D.M. Yourtee

University of Missouri-Kansas City, Schools of Pharmacy and Dentistry, 2411 Holmes Street, M4-209, Kansas City, MO 64108, USA

Many reports have demonstrated inflammation after the placement of dental restorations. To explain this side-effect, we studied a biomarker in the inflammatory response. The intercellular adhesion molecule-1 (ICAM-1) is a key mediator for recruitment of leukocytes to the site of inflammation. Therefore, we investigated whether methacrylates (a BISGMA-based dental resin, BISGMA, and MAA) and Cyracure^TM UVR 6105, an epoxy monomer, could alter ICAM-1 expression in unstimulated and TNF-a-stimulated endothelial cells. Six-well plates with monolayers of human umbilical vein cells, ECV 304 (ATCC CRL 1998), were exposed to TNF-a (1 ng/mL) in the presence and absence of subtoxic and TC₅₀ doses of chemicals for 24 hrs at 37°C/5% CO₂. Several doses of TNF-a (0.5-2 ng/mL) were co-incubated with 100 µL of undiluted aqueous dental resin extracts. Cells were harvested and stained with mAB FITC-conjugated anti-human ICAM-1 (CD54). ICAM-1 expression was measured by flow cytometry. Cells expressed basal levels of ICAM-1, which was up-regulated by TNF-a but was not changed by all samples studied. Except for UVR 6105, the methacrylates significantly decreased ICAM-1 expression in TNF--stimulated cells. These findings suggest that methacrylates may decrease the recruitment of leukocytes to sites of inflammation.

Key Words: endothelial cells • ICAM-1 expression • methacrylates • dental materials • TNF-a

Journal of Dental Research, Vol. 80, No. 9, 1789-1792 (2001)
DOI: 10.1177/00220345010800090301


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