This Article Free Full Text (Free PDF) Free References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Theilig, C. Articles by Geurtsen, W. Search for Related Content PubMed PubMed Citation Articles by Theilig, C. Articles by Geurtsen, W. Social Bookmarking                         What's this?

Effects of Mechanical Force on Primary Human Fibroblasts Derived from the Gingiva and the Periodontal Ligament

Department of Conservative Dentistry and Periodontology, Medical University Hannover, Carl-Neuberg-Str. 1, 30625 Hannover, Germany

A. Bernd

Department of Dermatology, JW Goethe University, Frankfurt, Germany

G. Leyhausen

Department of Conservative Dentistry and Periodontology, Medical University Hannover, Carl-Neuberg-Str. 1, 30625 Hannover, Germany

R. Kaufmann

Department of Dermatology, JW Goethe University, Frankfurt, Germany

W. Geurtsen

Department of Conservative Dentistry and Periodontology, Medical University Hannover, Carl-Neuberg-Str. 1, 30625 Hannover, Germany

Previous experiments have shown that mechanical stress may alter the interactions between cells and extracellular matrix (ECM). The purpose of our study was to investigate the effects of mechanical load on metabolism and ECM expression of primary human periodontal cells. The influence of gravitational force on proliferation, lactate dehydrogenase (LDH) release, and tenascin expression of gingival (HGF) and periodontal ligament fibroblasts (HPDL), as well as their adhesion to various extracellular matrix (ECM) components, was determined. Cells were centrifuged in microplates or flat tubes for 16 hrs at 217 g. Neither an enhanced release of LDH nor an alteration of cell proliferation could be detected after centrifugation. However, the attachment of loaded gingival and periodontal ligament fibroblasts to all tested ECM components significantly decreased in comparison with controls (Wilcoxon-Mann-Whitney test; HGF, p < 0.05; HPDL, p < 0.01). Tenascin expression of mechanically stressed fibroblasts significantly increased in comparison with controls (p < 0.01).

Key Words: periodontal fibroblasts • mechanical stress • tenascin • cell adhesion

Journal of Dental Research, Vol. 80, No. 8, 1777-1780 (2001)
DOI: 10.1177/00220345010800081901


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				T. A. H. Jarvinen, L. Jozsa, P. Kannus, T. L. N. Jarvinen, T. Hurme, M. Kvist, M. Pelto-Huikko, H. Kalimo, and M. Jarvinen Mechanical loading regulates the expression of tenascin-C in the myotendinous junction and tendon but does not induce de novo synthesis in the skeletal muscle J. Cell Sci., March 1, 2003; 116(5): 857 - 866. [Abstract] [Full Text] [PDF]

				J. Yang, W. C. Hooper, D. J. Phillips, M. L. Tondella, and D. F. Talkington Induction of Proinflammatory Cytokines in Human Lung Epithelial Cells during Chlamydia pneumoniae Infection Infect. Immun., February 1, 2003; 71(2): 614 - 620. [Abstract] [Full Text] [PDF]

				J. Yang, W. C. Hooper, D. J. Phillips, M. L. Tondella, and D. F. Talkington Centrifugation of Human Lung Epithelial Carcinoma A549 Cells Up-Regulates Interleukin-1{beta} Gene Expression Clin. Vaccine Immunol., September 1, 2002; 9(5): 1142 - 1143. [Full Text] [PDF]