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Osteoblast Integrin Adhesion and Signaling Regulate Mineralization

G.B. Schneider

Dows Institute for Dental Research and the Department of Prosthodontics, N402, University of Iowa College of Dentistry, Iowa City, IA 52242, galen-schneider{at}uiowa.edu

R. Zaharias

Dows Institute for Dental Research and the Department of Prosthodontics, N402, University of Iowa College of Dentistry, Iowa City, IA 52242

C. Stanford

Dows Institute for Dental Research and the Department of Prosthodontics, N402, University of Iowa College of Dentistry, Iowa City, IA 52242

Integrin adhesion and signaling events may contribute to the progressive differentiation of the osteoblast and to the initiation of a mineralized matrix. The purpose of our study was to begin to analyze the role of integrin receptors, in particular {alpha}2β1, {alpha}5β1, and {alpha}Vβ3, regarding mediation of the initiation of a mineralized matrix. Integrin-perturbation assays were conducted in microdot cultures of UMR-106-01 Bone Sialoprotein (BSP) osteoblast cells. For phenotypic analysis, we performed bright-field microscopy and Aliziran Red S staining to analyze effects on mineralization initiation. Mineralization was reduced significantly (P < 0.001) following the addition of a5- or β1-integrin subunit antibody by approximately 20% and 45%, respectively—{alpha}Vβ3 integrin by nearly 65%, and {alpha}2β1 integrin by nearly 95%. This effect was reversible following the removal of the anti-integrin antibody. These results suggest that integrin adhesion and signaling events may contribute to the ability of this cell line to mediate the initiation of the mineralization phenotype biologically.

Key Words: integrins • osteoblasts • mineralization • focal adhesions • implants • bone.

Journal of Dental Research, Vol. 80, No. 6, 1540-1544 (2001)
DOI: 10.1177/00220345010800061201
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