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Journal of Dental Research
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Mitogenic Effects of Neurotrophins on a Periodontal Ligament Cell Line

Y. Tsuboi

Department of Orthodontics

T. Nakanishi

Department of Biochemistry and Molecular Dentistry, Department of Biodental Research Center, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama 700-8525, Japan

T. Takano-Yamamoto

Department of Orthodontics

M. Miyamoto

Department of Orthodontics

T. Yamashiro

Department of Orthodontics

M. Takigawa

Department of Biochemistry and Molecular Dentistry, Department of Biodental Research Center, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama 700-8525, Japan, takigawa{at}md.okayama-u.ac.jp

Nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and neurotrophin-3 (NT-3) are three representative neurotrophins responsible for the differentiation and survival of neurons, and their high-affinity receptors are tropomyosin-receptor-kinase (TRK)A, TRKB, and TRKC, respectively. In this study, we investigated the expression of neurotrophins in a mouse periodontal ligament cell line (MPL), by reverse transcription-polymerase chain-reaction (RT-PCR) and enzyme-linked immunoabsorbent assay (ELISA). We also studied the expression of TRK receptors on MPL by immunostaining and the effects of neurotrophins on the proliferation of MPL, with a hypothesis of autocrine mechanism of neurotrophins. Each neurotrophin and TRK receptor was expressed, and neurotrophins enhanced the proliferation of MPL. These findings suggest that the MPL has functional neurotrophin receptors involved in an autocrine function of neurotrophins. The expression level of neurotrophins and TRKs showed the reverse pattern, and we propose an auto-regulatory mechanism of ligands and receptors in accordance with the level of synthesized neurotrophins.

Key Words: neurotrophin • tropomyosin-receptor-kinase (TRK) • periodontal ligament cell line • proliferation.

Journal of Dental Research, Vol. 80, No. 3, 881-886 (2001)
DOI: 10.1177/00220345010800030701
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