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Journal of Dental Research
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Acetylcholine-induced Vasodilation of Isolated Pulpal Arterioles

C.Y. Yu

School of Dentistry, The University of Western Australia, 179 Wellington Street, Perth, WA 6000, Australia, chrisyu{at}cyllene.uwa.edu.au

N.M. Boyd

School of Dentistry, The University of Western Australia, 179 Wellington Street, Perth, WA 6000, Australia

S.J. Cringle

Centre for Ophthalmology & Visual Science, The University of Western Australia, 2 Verdun Street, Nedlands, WA 6009

E.N. Su

Centre for Ophthalmology & Visual Science, The University of Western Australia, 2 Verdun Street, Nedlands, WA 6009

V.A. Alder

Biomedicine Division, Murdoch University, 90 South Street, Murdoch, WA 6150, Australia

D.Y. Yu

Centre for Ophthalmology & Visual Science, The University of Western Australia, 2 Verdun Street, Nedlands, WA 6009, Biomedicine Division, Murdoch University, 90 South Street, Murdoch, WA 6150, Australia

The presence of cholinergic mechanisms in the control of pulpal microcirculation has been a controversial issue. In this study, we aimed to determine the direct vasoactive responses of isolated pulpal arterioles to acetylcholine, and to investigate whether such responses are endothelium-dependent. Using an in vitro micro-perfusion system, we isolated pig pulpal arterioles, cannulated and perfused them intraluminally, and monitored the diameter. Following equilibration, the vessels were contracted with 10-5 M noradrenaline, and the effect of increasing doses of acetylcholine was determined. The influence of the muscarinic antagonist, atropine, or the loss of endothelial cell function following saponin treatment was also determined. Acetylcholine induced a dose-dependent vasodilation, reaching 94.6 ± 1.4% (n = 22) of the uncontracted diameter at 10-4 M. The vascular relaxation effect of acetylcholine was abolished in the presence of atropine, and by saponin treatment. Analysis of these data suggests that, in the pig, the acetylcholine-induced vasodilation of incisor pulpal arterioles is endothelium-dependent and mediated by muscarinic receptors.

Key Words: porcine incisor pulp • acetylcholine • endothelium-dependent vasodilation • isolated-perfused vessel.

Journal of Dental Research, Vol. 80, No. 11, 1995-1999 (2001)
DOI: 10.1177/00220345010800110901
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