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Journal of Dental Research
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High Glucose Suppresses Cathepsin Activity in Periodontal-ligament-derived Fibroblastic Cells

F. Nishimura

Department of Pcriodontology and Endodontology, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama 700-8525, Japan

K. Naruishi

Department of Pcriodontology and Endodontology, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama 700-8525, Japan

H. Yamada

Department of Pcriodontology and Endodontology, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama 700-8525, Japan

T. Kono

Department of Pcriodontology and Endodontology, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama 700-8525, Japan

S. Takashiba

Department of Pcriodontology and Endodontology, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama 700-8525, Japan

Y. Murayama

Department of Pcriodontology and Endodontology, Okayama University Dental School, 2-5-1 Shikata-cho, Okayama 700-8525, Japan, murayama{at}dent.okayama-u.ac.jp

The accumulation of extracellular matrices and integrins by high glucose has been reported in relation to diabetic complications. We previously reported that PDL cells expressed a higher amount of VLA-5 when cultured in high-glucose (4500 mg/L) medium than those cultured in low-glucose (1100 mg/L) medium. In this study, we aimed to address (1) whether this effect was mediated by the transcriptional level of the gene or the degradative level of the protein, and (2) whether this effect was mediated by TGF-β. The results indicated that the level of mRNA encoding a5 integrin did not change in PDL cells regardless of the concentration of glucose. Alternatively, high glucose suppressed cathepsin B+L activity. Additionally, the level of mRNA encoding TGF-β was not affected by high glucose, nor did an anti-TGF-β neutralizing antibody have an effect on the expression of β5 gene or cathepsin activity. Therefore, the effects of high glucose appeared to be mediated by impaired protein degradation, but not by autocrine TGF-β.

Key Words: hyperglycemia • VLA-5 • TGF-β • lysosomal enzyme • cathepsins.

Journal of Dental Research, Vol. 79, No. 8, 1614-1617 (2000)
DOI: 10.1177/00220345000790081501
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