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Journal of Dental Research
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Low Levels of GRP-Ca Expression in Transgenic Mice

K.G. Ten Hagen

Center for Oral Biology, Aab Institute of Biomedical Sciences, University of Rochester Medical Center, 601 Elmwood Avenue, Box 611, Rochester, NY 14642

M.M. Balys

Center for Oral Biology, Aab Institute of Biomedical Sciences, University of Rochester Medical Center, 601 Elmwood Avenue, Box 611, Rochester, NY 14642

The GRP-Ca gene of the rat encodes a member of the glutamine/glutamic-acid-rich protein (GRP) family. This gene is expressed in a highly tissue-specific fashion, with transcription being found only in the acinar cells of the submandibular gland (SMG). To begin to define the cis-acting elements governing GRP-Ca expression, we constructed transgenic mice containing the rat GRP-Ca gene plus 9.5 kb of 5' genomic sequence and 1 kb of 3' genomic sequence. Expression of GRP-Ca was detectable in progeny from only 1 of 3 independent founders. Expression levels of the transgenic GRP-Ca were much lower than the native GRP-Ca found in the rat SMG. Furthermore, GRP-Ca in transgenic mice was not tissue-specifically expressed, being found in both the SMG/SLG complex and the liver. These results indicate that the genomic region of GRP-Ca included in these transgenic mice is not sufficient to confer the high levels of tissue-specific expression seen in the rat.

Key Words: proline-rich proteins • glutamine/glutamic-acid-rich proteins • gene expression • submandibular gland.

Journal of Dental Research, Vol. 79, No. 4, 926-929 (2000)
DOI: 10.1177/00220345000790040701
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