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Prostaglandin E2 Predominantly Induces Production of Hepatocyte Growth Factor/Scatter Factor in Human Dental Pulp in Acute Inflammation

Department of Biochemistry

M. Suwa

Department of Operative Dentistry and Endodontology, Kagoshima University Dental School, 35-1 Sakuragaoka-8, Kagoshima, 890-8544 Japan

T. Oyama

Department of Operative Dentistry and Endodontology, Kagoshima University Dental School, 35-1 Sakuragaoka-8, Kagoshima, 890-8544 Japan

N. Arakaki

Department of Biochemistry

M. Torii

Department of Operative Dentistry and Endodontology, Kagoshima University Dental School, 35-1 Sakuragaoka-8, Kagoshima, 890-8544 Japan

Y. Daikuhara

Department of Biochemistry

Hepatocyte growth factor (HGF), which is also known as the scatter factor, is a broad-spectrum and multifunctional cytokine, mediates epithelial-mesenchyme interaction, and is shown to be involved in the development and regeneration of various tissues, including tooth. Here, we report that HGF was present in adult human dental pulps, and its levels increased during acute inflammation of the tissue. Levels of HGF mRNA in dental pulps also increased with inflammation, as determined by reverse-transcription/polymerase chain-reaction. The production of HGF in fibroblasts from dental pulps in culture was dose-dependently stimulated by inflammatory cytokines such as interleukin (IL)-la and tumor necrosis factor (TNF)-a, and by prostaglandin (PG) E2, as determined by an enzyme-linked immunosorbent assay. We also showed that indomethacin did not affect the increase in HGF production by the cells with IL-la, TNF-a, and PGE₂ . The levels of HGF mRNA in the cells were simultaneously increased by these stimulants, as determined by Northern blotting. Since the production of PGs is known to increase at the beginning of inflammation, PGE2 may be involved in the regeneration of dental pulps by the induction of HGF expression after inflammation.

Key Words: Hepatocyte growth factor • scatter factor • dental pulp • inflammation • prostaglandin • interleukin.

Journal of Dental Research, Vol. 79, No. 2, 748-755 (2000)
DOI: 10.1177/00220345000790020801


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