| Sign In to gain access to subscriptions and/or personal tools. |
Depressed Responsiveness of Peripheral Blood Mononuclear Cells to Heat-shock Proteins in Periodontitis PatientsAcademic Centre for Dentistry Amsterdam (ACTA), Department of Periodontology, Louwesweg 1, 1066 EA Amsterdam, The Netherlands
Academic Centre for Dentistry Amsterdam (ACTA), Department of General Pathology and Internal Medicine and Academic Medical Center, University of Amsterdam, Laboratory of Cellbiology and Histology, Amsterdam, The Netherlands
Academic Centre for Dentistry Amsterdam (ACTA), Department of Periodontology, Louwesweg 1, 1066 EA Amsterdam, The Netherlands
Institute of Infectious Diseases and Immunology, Veterinary Faculty, University of Utrecht, The Netherlands
Academic Centre for Dentistry Amsterdam (ACTA), Department of Periodontology, Louwesweg 1, 1066 EA Amsterdam, The Netherlands
The extensive homology between human and bacterial heat shock proteins (HSPs) may play a role in autoimmune reactions in periodontitis. Thus, we questioned whether peripheral blood mononuclear cell (PBMC) proliferative responses to HSPs are different between periodontitis patients and control subjects with gingivitis. The proliferative responses of PBMCs of patients (n = 10) and controls (n = 12) to recombinant mycobacterial HSP60 (MycHSP60) and HSP70 (MycHSP70), as well as recombinant human HSP60 (HumHSP60) and HSP70 (HumHSP70), were investigated. In addition, the proliferative responses to Candida albicans and purified protein derivatives of Mycobacterium (PPD) were included. Mean responses to HumHSP60, MycHSP60, and HumHSP70 were significantly lower for patients compared with controls. The responses to MycHSP70 showed a similar trend. However, when Candida and PPD were used as antigens, there was no difference in responses of the PBMCs between the periodontitis patients and controls. The level of IFN-
Key Words: periodontitis • heat shock protein • PBMC • T-lymphocytes • IFN-
Journal of Dental Research, Vol. 78, No. 8,
1393-1400 (1999) This article has been cited by other articles:
|
|
|||||||||||||||||||||||||||
 |
|
|
 |
|
Sign In to gain access to subscriptions and/or personal tools.
in the supernatants of the cells stimulated with HSPs was lower in the patients compared with controls. This concurs with the current hypothesis that periodontitis patients have a depressed Th1 response. Furthermore, we found that with an increasing estimated subgingival bacterial load, periodontitis patients mount a decreasing immune response to HSPs, while the controls showed a positive correlation between these two parameters. From these findings, we speculate that poor reactivity to HSPs may be a susceptibility factor for destructive periodontal disease and may need to be considered in the pathogenesis of this condition. 
